Sequences from confirmation amplicon


Confirmation sequences for line 108D09 and hit At3g54580 are shown.
Line ID 108D09
Locus At3g54580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20203670
Primers used for PCR IH57 - 3144, show primer details
   
Sequence Name 63-K100853-0022-108-D09C-3144
GenBank Accession LN510935 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K100853-0022-108-D09C-3144
TCCACTTCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTGAGGAATACAGTGGTGGTGGAGAGCTATCTGTGTATGG
ATCATATGCAGCAACCATTGTTGCCATGATGACAACTCCTAGAGCGCAAATGAGATGGGT
TGAAGGCCCCATCATAGAAGAAGATCTCATTGAGATTTTGTTTATTCTCTACCAACGCGT
TACTACGTTGGTTAGTTGTTTTTGTTTTGTTTTGTGTTGTAAAGAATGGGAGCATGGGGT
TTTATAGCAAAGTGTTTCTCAGAATCCAGCTCTACGATTATTTTTGCCCTGTCCTTGTGG
ACTCTGTCAATTAAATCGTGGAAGCTGGTCTACA
   
Sequence Name 63-K100859-0022-108-D09C-IH57
GenBank Accession LN510936 [GenBank]
Sequencing primer gene specific primer, IH57: 5'-GTGTAGACATGCTTCCACGATTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K100859-0022-108-D09C-IH57
GCAAAAAATCGTAGAGCTGGATTCTGAGAAACACTTTGCTATAAAACCCCATGCTCCCAT
TCTTTACAACACAAAACAAAACAAAAACAACTAACCAACGTAGTAACGCGTTGGTAGAGA
ATAAACAAAATCTCAATGAGATCTTCTTCTATGATGGGGCCTTCAACCCATCTCATTTGC
GCTCTAGGAGTTGTCATCATGGCAACAATGGTTGCTGCATATGATCCATACACAGATAGC
TCTCCACCACCACTGTATTCCTCACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATG
AAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATC
CCTTACGTCAGTGGAGATAT
Primers used for PCR 42wt - 8474, show primer details
   
Sequence Name 05-K118634-0022-108-D09C-8409
GenBank Accession LN488564 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K118634-0022-108-D09C-8409
GCCATTTACAATTGAATTATCCTGAGATTACGCTTCCTAAGATTGAATACAAAACTCCCC
CACTACCATACATCGACAGTTCCCCACCACCAACTTACTCACCGGCTCCAGAAGTAGAAT
ACAAATCTCCTCCACCACCATATGTTTACAGCTCACCACCACCACCGACCTACTCTCCAT
CCCCAAAGGTTGAGTACAAATCTCCTCCACCACCGTATGTCTACAGCTCCCCACCACCAC
CAACCTACTCTCCATCCCCAAAGGTTGAGTACAAATCTCCTCCACCACCCTATGTTTACA
GCTCCCCACCACCACCAACCTACTCTCCATCCCCAAAGGTTGAGTACAAATCTCCTCCAC
CACCGTACGTGTACAGCTCCCCACCACCACCAACCTACTCACCATCCCCAAAGGTTGAAT
ACAAATCTCCTCCACCACCGTACGTCTACAGCTCCCCACCACCACCAACCTACTCACCAT
CTCCAAAGGTTGAGTACAAGTCTCCTCCACCACCGTACGTCTACAGCTCTCCACCACCAC
CATACTACTCACCATCCCCAAAGGTAGAGTACAAGTCTCCTCCACCACCGTACGTCTACA
GCTCTCCACCACCACCATAATACTCTCCATCCCAAAAGGTAGATTACAAGTCTCCTCCAC
CGCCGTACGTCTACAGCTCTCCACCACCACCAAACTTACTCCCCATCCCCAAAGGTAGAT
TAA

Last Updated on 06.12.2018 16:11