Sequences from confirmation amplicon


Confirmation sequences for line 108F05 and hit At3g24503 are shown.
Line ID 108F05
Locus At3g24503 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8921990
Primers used for PCR 07fb - 3144, show primer details
   
Sequence Name 37-K110825-0022-108-F05C-07fb
GenBank Accession LN502650 [GenBank]
Sequencing primer gene specific primer, 07fb: 5'-CAAGAAGGACTCATGATTTGAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K110825-0022-108-F05C-07fb
ATTGATAACTTAGTTTTGGTTATTTGGGCTAAAATTAAAGGGAAGACGTTTGAGACGATA
GACCCTAGGAACGGTGAAGTGATCGCAACAATAGCCGAAGGAGACAAAGAAGACGTTGAC
TTGGCCGTTAACGCTGCACGTTACGCCTTCGACCATGGTCCTTGGCCTCGCATGACCGGC
TTCGTACGTTTCTTCTTCAACTCATGTTTTTTTTTATTGCACAACTACTCATTTTTTTCT
TCTTTAAATTATTGGTGATTTTAATTTTATTATTGTTGATTGATGTGGTCCCGTGGACAT
AGTGGATCATTAAATGTTTAGGGTCTTGTTCTGAGTGAATACTCTTTATTTGATTTGCTA
CGCAATTGGTAACTGTCGCAGCGTCGTGTTATTAAACTTATAGAAATGGAACGAGAGAAA
GTTGACTTATTTTGTAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACC
CTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGT
GGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACAATAATATAAT
TAAATTCACCTCTCATTTAAAGAAGAAAAAAATGAGTAGTTGTGCAATAGAAAAAAACAT
GAGTTGAAGAAG
   
Sequence Name 21-K110824-0022-108-F05C-3144
GenBank Accession LN495711 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K110824-0022-108-F05C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTACAAAATAAGTCA
ACTTTCTCTCGTTCCATTTCTATAAGTTTAATAACACGACGCTGCGACAGTTACCAATTG
CGTAGCAAATCAAATAAAGAGTATTCACTCAGAACAAGACCCTAAACATTTAATGATCCA
CTATGTCCACGGGACCACATCAATCAACAATAATAAAATTAAAATCACCAATAATTTAAA
GAAGAAAAAAATGAGTAGTTGTGCAATAAAAAAAAACATGAGTTGAAGAAGAAACGTACG
AAGCCGGTCATGCGAGGCCAAGGACCATGGTCGAAGGCGTAACGTGCAGCGTTAACGGCC
AAGTCAACGTCTTCTTTGTCTCCTTCGGCTATTGTTGCGATCACTTCACCGTTCCTAGGG
TCTATCGTCTCAAACGTCTTCCCTTTAATTTTTAGCCCAAATAAACCAAAACTAAGTTAT
CAATATATGTCAAATCAAACCATTTGGTTCAATGTTCAAATCATGAGTCCCTTC

Last Updated on 06.12.2018 16:11