Sequences from confirmation amplicon


Confirmation sequences for line 109A04 and hit At1g78680 are shown.
Line ID 109A04
Locus At1g78680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29595450
Primers used for PCR HP70 - 3144, show primer details
   
Sequence Name 67-K033553-022-109-A04C-3144
GenBank Accession LN511743 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K033553-022-109-A04C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGATTGAACTGG
AAGCCGGTCACTGGATATTTTGTTGATTGAACTGTTGAAACATAAACCTGGATATATGTT
TTAATAGATTTGGTCTATAAGGTCACAGGCTATTGAATCTTTTTAGGTATTCTTGGGGAC
ATAAAAATACCTTGCCGTTGTCATCAACGCATGTTGTGACAATTTTAAAGAAATTAGACA
GAGCAATATTTCCTTCAAAGCTTTGTGGTGAGATACCAAACTGGAAATAAGGACAATTCA
TATCAGAAAGCAGAGAGAACCGATGATAATAACCATTCAAGAACAAGAAATCAAAGAAGT
GGCAAGAAAAGTTGCTTAACTGCAGAGGTCAACAAAACTATGAGTATTGAATTGAATGTA
TAAGTATGTTTAGCAACAAAAATGGTGAAAACTATATTCCATAGTATTAACTGTAAAGAC
AAAGTACTGGTTACTAATACTACTAACCCGATGATCCTTGCATAA
   
Sequence Name 67-K033553-022-109-A04C-HP70
GenBank Accession LN511744 [GenBank]
Sequencing primer gene specific primer, HP70: 5'-GTTATGCAGAATCATCGGGTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K033553-022-109-A04C-HP70
TACTATGGAATATAGTTTTCACCATTTTTGTTGCTAAACATACTTATACATTCAATTCAA
TACTCATAGTTTTGTTGACCTCTGCAGTTAAGCAACTTTTCTTGCCACTTCTTTGATTTC
TTGTTCTTGAATGGTTATTATCATCGGTTCTCTCTGCTTTCTGATATGAATTGTCCTTAT
TTCCAGTTTGGTATCTCACCACAAAGCTTTGAAGGAAATATTGCTCTGTCTAATTTCTTT
AAAATTGTCACAACATGCGTTGATGACAACGGCAAGGTATTTTTATGTCCCCAAGAATAC
CTAAAAAGATTCAATAGCCTGTGACCTTATAGACCAAATCTATTAAAACATATATCCAGG
TTTATGTTTCAACAGTTCAATCAACAAAATATCCAGTGACCGGCTTCCAGTTCAATCAAA
CACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTC
TCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGT
GCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTT
GGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGG
Primers used for PCR FB12 - 8474, show primer details
   
Sequence Name 93-K034733-022-109-A04C-8409
GenBank Accession LN515226 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K034733-022-109-A04C-8409
GTCATTTTTAAATATCAACAAGTTAACTTTACGTCTCTCATTTAGTCTTTCACGCCTAGG
CATTGTCATTGTGGTCCACTGCGGTATCCTATCATCTATTAAAGAAAATCTCTGTACTAT
ACTCTGCAGAAAAATGCATTCGAGTGGGGATCATCTAAAATTCCACACTCTGAGGACGCG
ATCCAGGTGACTCAGCACGCCGCAAATCATCTAGTCAGGTTAGTTCTCTATCCATTTTTT
TGAAATAATTGATAAGAGAGTGCACCAACTCTGATTGCTACATTAGAATCAGTATAACTG
AGAGTTGGTTAGATCATGAAGTACTCATGGATCGTGTTTGAATGTGATGCAGTGAAGCTA
GGAAGTCACTGAACAGACCAGAGTCTAAGAAAGTGCTGAGCAATCTCATTTACAACTATA
AGCCTACTTACTGTGGATACGCAGGGTAAAGCCACTCTGACAATCTTTACATAATTCTCT
CTTCTTCGTCTTCTTCTTTTTTCTTCTAAGTTTTAACGATTCTTAGGATCGAATAAAATG
GTGGATTTTGCAGGATTGGTTACGACGA
   
Sequence Name 94-K034733-022-109-A04C-FB12
GenBank Accession LN515279 [GenBank]
Sequencing primer gene specific primer, FB12: 5'-ATGTAAACCTCGTCGTAACCAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K034733-022-109-A04C-FB12
ATCGTTAAAACTTAGAAGAAAAAAGAAGAAGACGAAGAAGAGAGAATTATGTAAAGATTG
TCAGAGTGGCTTTACCCTGCGTATCCACAGTAAGTAGGCTTATAGTTGTAAATGAGATTG
CTCAGCACTTTCTTAGACTCTGGTCTGTTCAGTGACTTCCTAGCTTCACTGCATCACATT
CAAACACGATCCATGAGTACTTCATGATCTAACCAACTCTCAGTTATACTGATTCTAATG
TAGCAATCAGAGTTGGTGCACTCTCTTATCAATTATTTCAAAAAAATGGATAGAGAACTA
ACCTGACTAGATGATTTGCGGCGTGCTGAGTCACCTGGATCGCGTCCTCAGAGTGTGGAA
TTTTAGATGATCCCCACTCGAATGCATTTTTCTGCAGAGTATAGTACAGAGATTTTCTTT
AATAGATGATAGGATACCGCAGTGGACCACAATGACAATGCCTAGGCGTGAAAGACTAAA
TGAGAGACGTAAAGTTAACTTGTTGATATTTAAAAATGACATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCCAAATGG

Last Updated on 06.12.2018 16:11