Sequences from confirmation amplicon


Confirmation sequences for line 109A09 and hit At3g24350 are shown.
Line ID 109A09
Locus At3g24350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8838561
Primers used for PCR TU07 - 8474, show primer details
   
Sequence Name 25-K026772-022-109-A09C-8409
GenBank Accession LN497287 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K026772-022-109-A09C-8409
GTAGATCTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCAACACCAGACAAGT
AAATCATAGCTCAGTCACGACTGCATACTCAAGCTTTATCGAAAAGAAATCGACGGAACA
AAGAAAAAACCTCGGTTCTCATGGTAAGAACATCCTTAAACTCTTTGGTAGTATCCATCA
AACGATACTTTAGATCGTCAACAACAGTTGCTGAGTGAGTACTTTTGTCCCTGTCTCTAG
AGTTATTCCCTTCATCATTCTGAGAACTGCGGAACAATTGAAGGTCGACCAGAGCAGAAT
TTAGAGCAGAGATCTCTTGCTTGATGACTACCGTCAGCTCTTGTATCTCCTGAGTAGGAT
CATCAAACACTGATGTCCTCTTTGCAACTGATTTGCAACAAAAACGAC
   
Sequence Name 26-K026772-022-109-A09C-TU07
GenBank Accession LN497762 [GenBank]
Sequencing primer gene specific primer, TU07: 5'-TGTTAACTTTGTCGTTTTTGTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K026772-022-109-A09C-TU07
TCAGTTGCAAAGAGGACATCAGTGTTTGATGATCCTACTCAGGAGATACAAGAGCTGACG
GTAGTCATCAAGCAAGAGATCTCTGCTCTAAATTCTGCTCTGGTCGACCTTCAATTGTTC
CGCAGTTCTCAGAATGATGAAGGGAATAACTCTAGAGACAGGGACAAAAGTACTCACTCA
GCAACTGTTGTTGACGATCTAAAGTATCGTTTGATGGATACTACCAAAGAGTTTAAGGAT
GTTCTTACCATGAGAACCGAGGTTTTTTCTTTGTTCCGTCGATTTCTTTTCGATAAAGCT
TGAGTATGCAGTCGTGACTGAGCTATGATTTACTTGTCTGGTGTTGCAGGATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
CATTCA

Last Updated on 06.12.2018 16:11