Sequences from confirmation amplicon


Confirmation sequences for line 109B06 and hit At3g06620 are shown.
Line ID 109B06
Locus At3g06620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2064611
Primers used for PCR EP01 - 3144, show primer details
   
Sequence Name 15-K023833-022-109-B06C-3144
GenBank Accession LN492789 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K023833-022-109-B06C-3144
GGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATT
TCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCCTCTACAT
GAAGCCATTGCTGATCCATGTAGAATTACTCTTTCTTCTTTCTTCTTTTGATTGTTTTTC
TCACTATTTGCAGTGGAAGTCTCTTCCGTCTACTACAGAAGAGCACGTCAAAACTGGATT
GGAGGCGGCGTATCCATATGGCCTTGGACATTGTAAGTATTAGTTGCACATTCACTTGAC
ATGTAACTTTTCCATTTCTATGAGCATTGGCAGTTGCATGTGGACAATGTTCTAAGTCTG
AGCTACTCTATTTGTTTACTTCTTTGTATTTGTCTTGTGATCATTCTTTTCCCCTCCTAT
TTGACTCAGTCAACGTACATCTCATCCAGCATATTACATGATTCTTCACACAGGCTCGCG
GTATGAATTATCTTCACCATTGTAGTCCACCCATTATCCATCGTGATCTGAAGTCATCAA
ATCTGCTGGTAGACAAGAACTGGACCGTTAAGGTAAGATATTCACCTTGAGACACTTTTA
CTTTCCTGTAATCTAGATGGATGATGATCAGTGAATCACTACATAGCTNTCTAAAACGAA
AATTTAACTTTAGAGAAATAAATCGTCTTATGTGAATGAACTGGAATAGTAAGACTCAGA
TC
   
Sequence Name 19-K023833-022-109-B06C-EP01
GenBank Accession LN494590 [GenBank]
Sequencing primer gene specific primer, EP01: 5'-AGTTCCATTTTGGAGATCTGAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K023833-022-109-B06C-EP01
TATTCCAGTTCATTCACATAAGACGATTTATTTCTCTAAAGTTAAATTTTCGTTTTAGAA
AGCTATGTAGTGATTCACTGATCATCATCCATCTAGATTACAGGAAAGTAAAAGTGTCTC
AAGGTGAATATCTTACCTTAACGGTCCAGTTCTTGTCTACCAGCAGATTTGATGACTTCA
GATCACGATGGATAATGGGTGGACTACAATGGTGAAGATAATTCATACCGCGAGCCTGTG
TGAAGAATCATGTAATATGCTGGATGAGATGTACGTTGACTGAGTCAAATAGGAGGGGAA
AAGAATGATCACAAGACAAATACAAAGAAGTAAACAAATAGAGTAGCTCAGACTTAGAAC
ATTGTCCACATGCAACTGCCAATGCTCATAGAAATGGAAAAGTTACATGTCAAGTGAATG
TGCAACTAATACTTACAATGTCCAAGGCCATATGGATACGCCGCCTCCAATCCAGTTTTG
ACGTGCTCTTCTGTAGTAGACGGAAGAGACTTCCACTGCAAATAGTGAGAAAAACAATCA
AAAGAAGAAAGAAGAAAGAGTAATTCTACATGGATCAGCAATGGCTTCATGTAGAGGAAG
GCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCT
TATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGT
Primers used for PCR FI61 - 8474, show primer details
   
Sequence Name 29-K028244-022-109-B06C-8409
GenBank Accession LN499152 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K028244-022-109-B06C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAAAAACAATCATTTCTGTAATTCAAA
TGATTATGGGCATAGATCAGAGGTATGGCATGAGGTCCAAAAGAAGAAAAACGAAAGTGA
GAGTAGTAACCAAAAGAGATGAGACCGAACCGTGGAAGGAACTCTGACACTATACAGAGA
CGCTGGGGTGAAGTCACAGCTCCCATAAACAGCAGGACGTTAGGGTGTCTAAGTCTTTTC
ATCAACAATACCTTTACAATAAAACAAGACCAACATAAATATTTGGAATTAGCTGTTTTC
GGAAATGATTCATTCTTCAGTAAGATGCAGTTCGTAAGTGAATATGCTTTAGTACAGAAA
ATTGTTGTCGTTGAAGAAGCAATCTGACTACTAAGAGATGAAAGAAAAAATCAATATTTT
GGTTATACTAAAAACCAATAAATATGTCAACTGTCTTATGCCAAAACTAAACTCACAGCA
CTG
   
Sequence Name 30-K028244-022-109-B06C-FI61
GenBank Accession LN499587 [GenBank]
Sequencing primer gene specific primer, FI61: 5'-TGTTGATTTATACGTGCATCCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K028244-022-109-B06C-FI61
GAAAAAAGGAATTCGCAAGTTCAAAAAATCTGTCTAATTAGGAGCTGCTAATGTCACCGC
AGGATGTAGCTGTAAAAGTGTTCTCCAAACAAGAATATTCAGCAGAGGTCATAGAATCTT
TTAAACAAGAGGTATTGTTTTTGATCTTTCTTACTAATCAGTGCTGTGAGTTTAGTTTTG
GCATAAGACAGTTGACATATTTATTGGTTTTTAGTATAACCAAAATATTGATTTTTTCTT
TCATCTCTTAGTAGTCAGATTGCTTCTTCAACGACAACAATTTTCTGTACTAAAGCATAT
TCACTTACGAACTGCATCTTACTGAAGAATGAATCATTTCCGAAAACAGCTAATTCCAAA
TATTTATGTTGGTCTTGTTTTATTGTAAAGGTATTGTTGATGAAAAGACTTAGACACCCT
AACGTCCTGCTGTTTATGGGAGCTGTGACTTCACCCCAGCGTCTCTGTATAGTGTCAGAG
TTCCTTCCACGGTTCGGTCTCATCTCTTTTGGTTACTACTCTCACTTTCGTTTTTCTTCT
TTTGGACCTCATGCCATACCTCTGATCTATGCCCATAATCATTTGAATTACAGAAATGAT
TGTTTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAG

Last Updated on 06.12.2018 16:11