Sequences from confirmation amplicon


Confirmation sequences for line 109G04 and hit At5g62230 are shown.
Line ID 109G04
Locus At5g62230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24998766
Primers used for PCR SD03 - 8474, show primer details
   
Sequence Name 21-K023014-022-109-G04C-8409
GenBank Accession LN495491 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K023014-022-109-G04C-8409
TTTCCCGGACATGAAGCCATTTACATTCTTGAATTTGGCTCGTCTGACTTTGTGAATCCT
ATTCAATTCACAGGGATTTGAGTGACAATGAGCTTGTTGGTCCTATCCCACCGATACTTG
GCAATCTCTCATTTACCGGAAAGTTGTGAGTCTTTTTTGTATAACTGTAGTATCTCATAT
GTTCAGCTAATCAGCTCTTGGCATATGCATTGATACCTTTTTTTTTTGTTGAAATAGGTA
TCTCCATGGCAATATGCTCACTGGTCCAATCCCCTCTGAGCTTGGGAATATGTCACGTCT
CAGCTATTTGTAAGCTTGTTTTAAACTTCAAATCTGTTTACATAGCTTTCG
   
Sequence Name 22-K023014-022-109-G04C-SD03
GenBank Accession LN495938 [GenBank]
Sequencing primer gene specific primer, SD03: 5'-TATGGAATGCGAAAGCTATGTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K023014-022-109-G04C-SD03
CAGACTTGAAGTTTAAAACAAGCTTACAAATAGCTGAGACGTGACATATTCCCAAGCTCA
GAGGGGATTGGACCAGTGAGCATATTGCCATGGAGATACCTATTTCAACAAAAAAAAAAG
GTATCAATGCATATGCCAAGAGCTGATTAGCTGAACATATGAGATACTACAGTTATACAA
AAAAGACTCACAACTTTCCGGTAAATGAGAGATTGCCAAGTATCGGTGGGATAGGACCAA
CAAGCTCATTGTCACTCAAATCCCTGTGAATTGAATAGGATTCACAAAGTCAGACGAGCC
AAATTCAAGAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11