Sequences from confirmation amplicon


Confirmation sequences for line 110B11 and hit At4g25030 are shown.
Line ID 110B11
Locus At4g25030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12866595
Primers used for PCR CD58 - 8474, show primer details
   
Sequence Name 28-K104189-0022-110-B11C-CD58
GenBank Accession LN498820 [GenBank]
Sequencing primer gene specific primer, CD58: 5'-CCGCTTAATTGTTGTTTCTCTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K104189-0022-110-B11C-CD58
GGCTTAAACAGGAAACTGCCCAATAGCAAAATCATATCGAGCTGTAAGTGGTGTGGCACC
ACTTGTGGCAAAGATCCTGCAACCCCCTCCAGGCATGAAATTTAAATGCCCTCAAGCAAT
AGTCACAGCTCGAGCAGCGATATCAAAAACACCTTTCGCCAAGAACCTCCGTCCACAACC
TTTACCAGCTAAAGTACTAGTGATCGGGATGCTGGGCATGGCGTTAAATGTGCCTTTAGG
GGTCTGGAGAGAGCACACTGAAAAGTTTTCGGCATCTTGGTTTATAGCTCTTCACGCAGC
GGTTCCATTCATAGGAATACTGAGAAAGTCAGTGTTGATGCCTAAAACAGCTATGGTATT
CACCATAGCAGCATCAGTTTTGGGACAGGTGATTGGGTCAAGAGCAGAGAGGCGGAGACT
CAAGTCTGTAGCTGAGAAGAAGTTGACATTGGAGGTACCAAATCCGTCTTCTGTTGAAGC
AGATCAGATGCAATTTGCCGGAGTTTCATCAGATGGAAGATGCGGTGATAAAGTGGTGAT
GAAGTGGAATCCCATGATGCTTGATGTTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 44-K104190-0022-110-B11C-8409
GenBank Accession LN505169 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K104190-0022-110-B11C-8409
CCATTTACACATCAAGCATCATGGGATTCCACTTCATCACCACTTTATCACCGCATCTTC
CATCTGATGAAACTCCGGCAAATTGCATCTGATCTGCTTCAACAGAAGACGGATTTGGTA
CCTCCAATGTCAACTTCTTCTCAGCTACAGACTTGAGTCTCCGCCTCTCTGCTCTTGACC
CAATCACCTGTCCCAAAACTGATGCTGCTATGGTGAATACCATAGCTGTTTTAGGCATCA
ACACTGACTTTCTCAGTATTCCTATGAATGGAACCGCTGCGTGAAGAGCTATAAACCAAG
ATGCCGAAAACTTTTCAGTGTGCTCTCTCCAGACCCCTAAAGGCACATTTAACGCCATGC
CCAGCATCCCGATCACTAGTACTTTAGCTGGTAAAGGTTGTGGACGGAGGTTCTTGGCGA
AAGGTGTTTTTGATATCGCTGCTCGAGCTGTGACTATTGCTTGAGGGCATTTAAATTTCA
TGCCTGGAGGGGGTTGCAGGATCTTTGCCACAAGTGGTGCCACACCACTTACAGCTCGAT
ATGATTTTGCTATTGGGCAGTTTCCTGTTTTAAGCCATTCGTCGCCCATGGCTTCGTGGT
TTCCTCCCTGAAAGAGAAACAACAATTTAAGCGG

Last Updated on 06.12.2018 16:11