Sequences from confirmation amplicon


Confirmation sequences for line 110C12 and hit At3g03340 are shown.
Line ID 110C12
Locus At3g03340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:788613
Primers used for PCR 09fb - 8474, show primer details
   
Sequence Name 35-K110825-0022-110-C12C-09fb
GenBank Accession LN501869 [GenBank]
Sequencing primer gene specific primer, 09fb: 5'-CGATCATAATCTCTTCGATGTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K110825-0022-110-C12C-09fb
TTGATTCTCTCTCCTTACTGTTCCGTTCCTTTGATCTAAGTATGGTTTTAAGTATTCACT
ATGAAGTCAATTTCAAATCAACACCGTAATAAAACAATGAAACAAGAAGCTAGAACTAAA
ACCTAACCTTTCCTTGCGTATATTGGCCTTCTCATCCTGAAGTAAACGAAGAGAAATATA
AGAAGGCAGTTTCATCCATCATCAGATAAAGTAGATTGTGTTATTAGTTTACCAGAAGCT
CTGTTAATTTGTCCCGGACTAGCATGTATCCTAGATGAAGCTTCCCCCCAAAATGATCAG
CTAAACGGCGATCACTGTAGCATCACAAAAGTTCCAATTAGCATAGCCAAATGCTAAACA
TTTGACGATAGCATATTAAAACAGTATAACGTTAAAAACTCCATTAGTTAATCTCTACTA
GTAAGATCCTTCAAGCACAATAAAATCAAGAAAAGAACGCACATTTATTATAGCTGCAAA
AGTAATCCTGCCCAAAAAGCCTGCTGGTGAAAGGAGGAGGTACAATAGCCGGAGTCAAAA
GACTATTTCTACCTTTTTTCTCTTTCAATCCAAAAGGGAAACAATAATGCAGAAAGTTTG
AGCAAGGAACTTAACCTGTCATAGACGCTCAAGAATGCTCCACATATGTCACATAGACGC
AACTTTTGGTCTGTCTGAGAAGATATAGGAACAATAAGTGAGGTTAGAAGATGAAACCAA
TGTGTAGATACATTAAACAGGGGATGCGAAGATGTTATACTTTTGGTCCAAATAGTAACT
TTTATGAAAGCAATCAAACGGGTTTACTCAAATAAACTATTACTCTTTACCTAAACGTTA
ATATATTTCACGGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGGAGAAAAGAAAGAGTAATTACCAATTTTTTTTCATTCAAAAAT
   
Sequence Name 19-K110824-0022-110-C12C-8409
GenBank Accession LN494809 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K110824-0022-110-C12C-8409
GCCATTTACCGTTGAATTATTAACGTTTAGGTAAAGAGTAATAGTTTATTTGAGTAAACC
CGTTTGATTGCTTTCATAAAAGTTACTATTTGGACCAAAAGTATAACATCTTCGCATCCC
CTGTTTAATGTATCTACACATTGGTTTCATCTTCTAACCTCACTTATTGTTCCTATATCT
TCTCAGACAGACCAAAAGTTGCGTCTATGTGACATATGTGGAGCATTCTTGAGCGTCTAT
GACAGGTTAAGTTCCTTGCTCAAACTTTCTGCATTATTGTTTCCCTTTTGGATTGAAAGA
GAAAAAAGGTAGAAATAGTCTTTTGACTCCGGCTATTGTACCTCCTCCTTTCACCAGCAG
GCTTTTTGGGCAGGATTACTTTTGCAGCTATAATAAATGTGCGTTCTTTTCTTGATTTTA
TTGTGCTTGAAGGATCTTACTAGTAGAGATTAACTAATGGAGTTTTTAACGTTATACTGT
TTTAATATGCTATCGTCAAATGTTTAGCATTTGGCTATGCTAATTGGAACTTTTGTGATG
CTACAGTGATCGCCGTTTAGCTGATCATTTTGGGGGGAAGCTTCATCTAGGATACATGCT
AGTCCGGGACAAATTAACAGAGCTTCTGGTAAACTAATAACACAATCTACTTTATCTGAT
GATGGATGAAACTGCCTTCTTATATTTCTCTTCGTTTACTTCAGGATGAGAAGGCCAATA
TACGCAAGGAAAGGTTAGGTTTTAGTTCTAGCTTCTTGTTTCATTGTTTTATTACGGTGT
TGATTTGAAATTGACTTCATAGTGAATACTTAAAACCATACTTAGATCAAAGGAACGGAA
CAGTAAGGAGAGAGAATCAAGTTAAGACCGAGAGAAAGAACAGAAACTAGTCGTGAACAT
CG

Last Updated on 06.12.2018 16:11