Sequences from confirmation amplicon


Confirmation sequences for line 110D04 and hit At2g05715 are shown.
Line ID 110D04
Locus At2g05715 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:2146969
Primers used for PCR ME14 - 8474, show primer details
   
Sequence Name 26-K116478-0022-110-D04C-ME14
GenBank Accession LN498085 [GenBank]
Sequencing primer gene specific primer, ME14: 5'-CATCCACAGACGAGAACACTACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K116478-0022-110-D04C-ME14
CTTTAGACACAATCTTGTTTGTAACTTGAGGCACCTCCCACAATTTAGGAACCCCACTAA
GAGAACATGTGACGAGTATTTTTCCATCTCGTGATAAGGAGCAACCAGTAAGAGGACGAT
CATCCCCAAATTTACTAGAACCAAGAACCATGTCTTTACAATGCTTTAAAGCCAATTTAG
TCTCAGCATTTTTATCTTCATCTGGATCATTCCTTCGCCGATTTTCACGCTGAATCCTTA
GAGCAGCTCTCTTTATAAAATCTTTGGTAATTTCTATTCTAGCCTCTCTAAGCTCTGTTG
GACCCATTGGTATAAACAAACTTTTGAAACAAGATCTAGAAGAAGCTCGTGACAAGACTA
GTTCACTTAGAAGACAAGAAGGTTTGGCTTACCAAAGCTCTTATTTATAGCAAAGTAAAC
GAGATGGATCAGAAAGAAAGAAAGGAAATCTATTAAAAAAGTGGGTTATTGGGGTTAGCT
TTGAATTGTGATTTGTCTCTTAAGTCTAAATTCGATAGGAAGCAGTATTTCGCAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCG
   
Sequence Name 01-K116477-0022-110-D04C-8409
GenBank Accession LN486731 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K116477-0022-110-D04C-8409
ATTGAATATATCCTGCGAAATACTGCTTCCTATCGAATTTAGACTTAAGAGACAAATCAC
AATTCAAAGCTAACCCCAATAACCCACTTTTTTAATAGATTTCCTTTCTTTCTTTCTGAT
CCATCTCGTTTACTTTGCTATAAATAAGAGCTTTGGTAAGCCAAACCTTCTTGTCTTCTA
AGTGAACTAGTCTTGTCACGAGCTTCTTCTAGATCTTGTTTCAAAAGTTTGTTTATACCA
ATGGGTCCAACAGAGCTTAGAGAGGCTAGAATAGAAATTACCAAAGATTTTATAAAGAGA
GCTGCTCTAAGGATTCAGCGTGAAAATCGGCGAAGGAATGATCCAGATGAAGATAAAAAT
GCTGAGACTAAATTGGCTTTAAAGCATTGTAAAGACATGGTTCTTGGTTCTAGTAAATTT
GGGGATGATCGTCCTCTTACTGGTTGCTCCTTATCACGAGATGGAAAAATACTCGTCACA
TGTTCTCTTAGTGGGGTTCCTAAATTGTGGGAGGTGCCTCAAGTTACAAACAAGATTGTT
GTCTTGAAAGGTCATAAAGAACATGTAACTGATGTAGTGTTCT
Primers used for PCR 05ub - 8474, show primer details
   
Sequence Name 18-K117104-0022-110-D04C-05ub
GenBank Accession LN494478 [GenBank]
Sequencing primer gene specific primer, 05ub: 5'-TCAGCAGCATCTATACCAATTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K117104-0022-110-D04C-05ub
ATCACTTGCACCATTAGAGTAACCAACTAATACATAAGAAACACAAACATCTTGCAGCTG
AAGCCTAAAACCAATACAAGAATCTGATAAAACCAAACTGAAACTTGTCATAAATTCCAT
ATGAACCTGAGAGTAATCTCCTGTGTCTGAATGAAGTAACTTGCCAGCATCTTCTTCCAT
ATGAACCCTAGTAATACCAAAATCTCCTATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCG
   
Sequence Name 02-K0117103-0022-110-D04C-8409
GenBank Accession LN486810 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K117103-0022-110-D04C-84O9
TGGTATACTAGGGTTCATATGGAAGAAGATGCTGGCAAGTTACTTCATTCAGACACAGGA
GATTACTCTCAGGTTCATATGGAATTTATGACAAGTTTCAGTTTGGTTTTATCAGATTCT
TGTATTGGTTTTAGGCTTCAGCTGCAAGATGTTTGTGTTTCTTATGTATTAGTTGGTTAC
TCTAATGGTGCAAGTAGATTTGAATAGAGCAGGTGTTCTTGAACATGATCTGAGAATTGG
TATAAATGCTGCTGAA

Last Updated on 06.12.2018 16:11