Sequences from confirmation amplicon


Confirmation sequences for line 110D04 and hit At3g55090 are shown.
Line ID 110D04
Locus At3g55090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20416392
Primers used for PCR MZ30 - 8474, show primer details
   
Sequence Name 35-K027355-022-110-D04C-8409
GenBank Accession LN501694 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K027355-022-110-D04C-8409
CATTTCATTTAAAACAGAGGAGGTGAAACTGAAACTACTGATGATACCTAAAACCAAAAA
CATATATTGTAATTCTTTTTCATTACTTGTAATTCGTAGATTGATTGTGTATTTGAATTG
TATTCTTTTTAACATTTGTATTACTTTTGCCATCAATAAAAAAAATATCATTCTTTAGGA
AATTGGAAATACAAAAAGCACCTATGAATCGCTTTTACTCAGAAAATTAATGCAACATTT
CAAATAGAGGCATTGAGATTGTGGAAGAAAAATGAGGAGACACAGACACTGTTGCTAGAG
GCAAGTAACTCTCAAAGCCATTTCACTTTGTAAGGTATCAAAAAGTCAAAATTGGAAGGC
TATCTACAAAATCAATTAAAAACAAACCGATTTATCTGAAATTTAAAACCGAAACAAACC
GGACCGAATCAGCATCAGAATATCGTCTGGTCCTGGCCGCTCTGCTCTAGCCGCAGTCGA
AACACGAGTCCATAATACGAGCGGTGGTTGCTCACGCGCCATCCAGTTTGGCTGGAGATG
CTATTCTGCATTTCGACAGTGAAGCTTTTCGGGTTTCAACAAAGGCGCAATGTTAGCTCT
TTAGCCAAGCGTTTCAGTCTCGCCGGGAAACTAACGTTAGAGCTCCAAACTCAGGTTTGA
TTTGTGAAAGATTGAGACTTTCGTGAAATTTGTGAAATCAATGTGATGGACTGTTCACAG
CTTGATTATCTCAAGTGTTAGAAAAGTTTTATCATACCTGAGTG
   
Sequence Name 36-K027355-022-110-D04C-MZ30
GenBank Accession LN502086 [GenBank]
Sequencing primer gene specific primer, MZ30: 5'-AGAAAAATTCTTGGAACCACACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K027355-022-110-D04C-mz30
GCTGTGAAAGTCCTCCATTGATTTCACAAATTTCACGAAAGTCTCAATCTTTCACAAATC
AAACCTGAGTTTGGAGCTCTAACGTTAGTTTCCCGGCGAGACTGAAACGCTTGGCTAAAG
AGCTAACATTGCGCCTTTGTTGAAACCCGAAAAGCTTCACTGTCGAAATGCAGAATAGCA
TCTCCAGCCAAACTGGATGGCGCGTGAGCAACCACCGCTCGTATTATGGACTCGTGTTTC
GACTGCGGCTAGAGCAGAGCGGCCAGGACCAGACGATATTCTGATGCTGATTCGGTCCGG
TTTGTTTCGGTTTTAAATTTCAGATAAATCGGTTTGTTTTTAATTGATTTTGTAGATAGC
CTTCCAATTTTGACTTTTTGATACCTTACAAAGTGAAATGGCTTTGAGAGTTACTTGCCT
CTAGCAACAGTGTCTGTGTCTCCTCATTTTTCTTCCACAATCTCAATGCCTCTATTTGAA
ATGTTGCATTAATTTTCTGAGTAAAAGCGATTCATAGGTGCTTTTTGTATTTCCAATTTC
CTAAAGAATGATATTTTTTTTATTGATGGCAAAAGTAATACAAATGTTAAAAAGAATACA
ATTCAAATACACAATCAATCTACGAATTACAAGTAATGAAAAAGAATTACAATATATGTT
TTTGGTTTTAGGTATCATCAGTAGTTTCAGTTTCACCTCCTCTTGTTTTTAAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
ANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAANTAACAAATTATTCTAATTC
Primers used for PCR 07ub - 8474, show primer details
   
Sequence Name 20-K117104-0022-110-D04C-07ub
GenBank Accession LN495383 [GenBank]
Sequencing primer gene specific primer, 07ub: 5'-GATTCATCTTAGCCACCGTATTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K117104-0022-110-D04C-07ub
GGTTAGGGTTCTTCGCATTCGCAATGTCAACAATGTTCTACACTTGCGCCGATGCTCTTC
CCGTGTTCCTCCAGGAACGTTACATCTTCATGAGAGAAACAGCTTACAACGCTTACCGGA
GATCCTCCTACGTTCTCTCTCACGCCATTGTTACCTTCCCTTCGCTCATCTTCCTCTCTT
TAGCTTTCGCAGTGACGACGTTTTGGGCTGTTGGGCTTGAAGGAGGCCTAATGGGCTTTT
TATTCTACTGCTTAATCATCTTAGCCTCTTTCTGGTCCGGAAGCTCCTTCGTGACTTTCT
TATCCGGCGTCGTTCCACATGTGATGTTAGGCTACACGATCGTTGTTGCTATTTTAGCTT
ACTTCTTGCTTTTCAGTGGCTTCTTCATCAACAGAGATCGAATCCCTCAGTATTGGATTT
GGTTTCATTATCTTTCTCTGGTTAAGTATCCATACGAGGCGGTTCTTCAGAACGAGTTCT
CGGATCCTACAGAATGTTTTGTGAGAGGTGTTCAGCTTTTCGACAACTCGCCTTTGGGAG
AATTGACGTATGGGATGAAGCTGAGGCTTTTGGATTCTGTGAGCCGGTCGATAGGTATGA
GGATATCGAGCTCTACGTGTTTAACGACGGGTGCTGATGTTCTGAAGCAGCAAGGAGTGA
CACAGCTTAGTAAATGGAACTGCTTGCTTATCACGGTAGGTTTCGGATTCCTATTTAGGA
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTAAAAATGTA
G
   
Sequence Name 04-K117103-0022-110-D04C-OriL
GenBank Accession LN488082 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K117103-0022-110-D04C-0riL
ATTTACATTGAATATATTCCTAAATAGGAATCCGAAACCTACCGTGATAAGCAAGCAGTT
CCATTTACTAAGCTGTGTCACTCCTTGCTGCTTCAGAACATCAGCACCCGTCGTTAAACA
CGTAGAGCTCGATATCCTCATACCTATCGACCGGCTCACAGAATCCAAAAGCCTCAGCTT
CATCCCATACGTCAATTCTCCCAAAGGCGAGTTGTCGAAAAGCTGAACACCTCTCACAAA
ACATTCTGTAGGATCCGAGAACTCGTTCTGAAGAACCGCCTCGTATGGATACTTAACCAG
AGAAAGATAATGAAACCAAATCCAATACTGAGGGATTCGATCTCTGTTGATGAAGAAGCC
ACTGAAAAGCAAGAAGTAAGCTAAAATAGCAACAACGATCGTGTAGCCTAACATCACATG
TGGAACGACGCCGGATAAGAAAGTCACGAAGGAGCTTCCGGACCAGAAAGAGGCTAAGAT
GATTAAGCAGTAGAATAAAAAGCCCATTAGGCCTCCTTCAAGCCCAACAGCCCAAAACGT
CGTCACTGCGAAAGCTAAAGAGAGGAAGATGAGCGAAGGGAAGGTAACAATGGCGTGAGA
GAGAACGTAGGAGGATCTCCGGTAAGCGTTGTAAGCTGTTTCTCTCATGAAGATGTAACG
TTCCTGGAGGAACACGGGAAGAGCATCGGCGCAAGTGTAGAACATTGTTGACATTGCGAA
TGCGAAGAACCCTAACCGCTCTTGAACTCCTTTTGGTGAATTATCCAATCGCCAGAATAC
GGTG

Last Updated on 06.12.2018 16:11