Sequences from confirmation amplicon


Confirmation sequences for line 110D04 and hit At5g15240 are shown.
Line ID 110D04
Locus At5g15240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4948954
Primers used for PCR UF06 - 3144, show primer details
   
Sequence Name 31-K027353-022-110-D04C-3144
GenBank Accession LN500016 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K027353-022-110-D04C-3144
ATCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGA
CAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGA
AATATCACGGTAGGTTAGGCTTGGACATTCTTCTGATCAACTCTTTTTTTTTTAGGTGTG
GGTATATTATCGGTCCCTTATGCCTTAGCATCCGGAGGATGGCTAAGTTTGATCATACTT
TTCACCGTAGCGATCACAACTTTCTACTGTGCCATTCTCATTAAGAGATGTATGGAAATG
GATCCACTTCTTAGATCATACCCGGACATTGGCTACAAAGCCTTTGGAAACACAGGACGT
GTTATCGTCTCCATCTTCATGAACCTCGAGCTTTACCTAGTTGCAACTAGTTTCTTGATC
CTAA
   
Sequence Name 32-K027353-022-110-D04C-UF06
GenBank Accession LN500437 [GenBank]
Sequencing primer gene specific primer, UF06: 5'-AGGTTGTCACCTTCTAGGATCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K027353-022-110-D04C-uf6
AAGATGGAGACGATAACACGTCCTGTGTTTCCAAAGGCTTTGTAGCCAATGTCCGGGTAT
GATCTAAGAAGTGGATCCATTTCCATACATCTCTTAATGAGAATGGCACAGTAGAAAGTT
GTGATCGCTACGGTGAAAAGTATGATCAAACTTAGCCATCCTCCGGATGCTAAGGCATAA
GGGACCGATAATATACCCACACCTAAAAAAAAAAGAGTTGATCAGAAGAATGTCCAAGCC
TAACCTACCGTGATATTTCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCT
GATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTC
TTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATC
CACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGT
Primers used for PCR 04ub - 8474, show primer details
   
Sequence Name 17-K117104-0022-110-D04C-04ub
GenBank Accession LN494029 [GenBank]
Sequencing primer gene specific primer, 04ub: 5'-TGCATGTTTTTAACTCAAGCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K117104-0022-110-D04C-04ub
TACATTTTGTTTCTTACCTCCTAGTCTTGCCTCAGTTTTTTTAGTTATTTAATTTTTCTG
TTGATAATTTATCATAACCAAGTTAAAACCAGTTCTACTTAGACTGAATTGTGTTTTTCT
TCGTCTAGATTCAGTTTCATAGTCTTTGATAAACAATAGTGCTAAATAACCAAATCATTT
TTGTTCTGACTATTAAAGGTTAGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGC
   
Sequence Name 01-K117103-0022-110-D04C-8409
GenBank Accession LN486747 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K117103-0022-110-D04C-8409
CTACCTTTATAGTCAGAACAAAAATGATTTGGTTATTTAGCACTATTGTTTATCAAAGAC
TATGAAACTGAATCTAGACGAAGAAAAACACAATTCAGTCTAAGTAGAACTGGTTTTAAC
TTGGTTATGATAAATTATCAACAGAAAAATTAAATAACTAAAAAAACTGAGGCAAGACTA
GGAGGTAAAGAAACAAAATGTAGGATTGAAATACACACCAATCTAGATGATACAAGATAA
AGTCAAAAGCTTGAGTAAAAAACATGC

Last Updated on 06.12.2018 16:11