Sequences from confirmation amplicon


Confirmation sequences for line 110H12 and hit At2g41620 are shown.
Line ID 110H12
Locus At2g41620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17352949
Primers used for PCR SR39 - 8474, show primer details
   
Sequence Name 71-K103592-0022-110-H12C-SR39
GenBank Accession LN512545 [GenBank]
Sequencing primer gene specific primer, SR39: 5'-CAATCATATGGTCCTGAAAAGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K103592-0022-110-H12C-SR39
TCCAAACCTGTTGCCAGTAGTATCAACAGGAGGCTGCCTACGAGCATCAGTTGAATCAAA
ATCTAAAACTCCATAGTCTCTCAAACGAATCTGCACAGTATAGATAACATGAAAATAAGA
AAGCTAAACAGCCCTATGAACATTTTCTGTGTGGTTTCAATCTGGGACACTAACCCGAAG
GAAAGCACGAATTCTTTGCAAATTTCCAACAGATCCACCAAGAGCAGCCTAGAACAGGAA
TATAAAAAGCAAGAAACATGTCAAAATGATTACCAACATTCTTGAAATAGGATTGAAAGA
TCATGGAACCTTTTCAGACAGCACCCAACCATTTGAGGCAGAGGATAATCAAAATATGTA
TTGACATACAAGTCTTAACTCTTCTTATTAAGTATACAAAAAATGGATACCTGTGTAGGG
TGAGTTTGAATTGTGTCCATTATAAATTTCTCATGCCCATGTTGTAAATGATGTCTTGCA
CCTATCGCGAGGGCCATCCTCTTCGAAACACCCTGTCGAACTGCTGAATCTTCACCTGTT
ATTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTNCATT
CAAAAATGTAGATGTCCGCA
   
Sequence Name 65-K103593-0022-110-H12C-8409
GenBank Accession LN511381 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K103593-0022-110-H12C-8409
TTACAATTGAATATATCCTGACAATAACAGGTGAAGATTCAGCAGTTCGACAGGGTGTTT
CGAAGAGGATGGCCCTCGCGATAGGTGCAAGACATCATTTACAACATGGGCATGAGAAAT
TTATAATGGACACAATTCAAACTCACCCTACACAGGTATCCATTTTTTGTATACTTAATA
AGAAGAGTTAAGACTTGTATGTCAATACATATTTTGATTATCCTCTGCCTCAAATGGTTG
GGTGCTGTCTGAAAAGGTTCCATGATCTTTCAATCCTATTTCAAGAATGTTGGTAATCAT
TTTGACATGTTTCTTGCTTTTTATATTCCTGTTCTAGGCTGCTCTTGGTGGATCTGTTGG
AAATTTGCAAAGAATTCGTGCTTTCCTTCGGGTTAGTGTCCCAGATTGAAACCACACAGA
AAATGTTCATAGGGCTGTTTAGCTTTCTTATTTTCATGTTATCTATACTGTGCAGATTCG
TTTGAGAGACTATGGAGTTTTAGATTTTGATTCAACTGATGCTCGTAGGCAGCCTCCTGT
TGATACTACTTGGCAACAGGTTTTGGATTATCTCCCTCTTGCTTTGAATTTTAAACCTTT
TCAGGACATAATGATTG

Last Updated on 06.12.2018 16:11