Sequences from confirmation amplicon


Confirmation sequences for line 111B05 and hit At3g27980 are shown.
Line ID 111B05
Locus At3g27980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10395026
Primers used for PCR IH61 - 8474, show primer details
   
Sequence Name 93-K100855-0022-111-B05C-8409
GenBank Accession LN515233 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K100855-0022-111-B05C-8409
CATTTACAATTGAATATATCCTGTTAAGAAGAACGTACAACACCGCAACTGTTGGTAAGA
ATTTGTATTCTCTTCTTGAATAGTTTATAGTTTTCTCTTCTTTATACCAAACTATAACAT
TCTTAAAGACATTACTAATTATTCTAAAAATATGTTTGAAGCTTCTAATGGTAATGGATT
CATCGGAGTAGACATGTGCTTTCGAAACACGGCCGGACCGGCAAAAGGACCAGCTGTTGC
CCTCCGTGTGAGTGGTGATATGTCCGTCATTTATAGATGTCGTGTCGAGGGATATCAGGA
TGCCTTGTATCCTCATAGTGACCGCCAGTTTTATAGGGAGTGCTTTATTACCGGCACTGT
AGATTTTATATGTGGAAACGCGGTGGCGGTCTTTCAATTCTGCCAGATTGTAGCAAGACA
GCCTAAGATGGGGCAGAGCAATGTCATAACCGCTCAATCACGCGCGACTAAGGACGTCAA
ATCGGGCTTCTCAATTCAAAACTGCAACATCCAAACAAAGTT
   
Sequence Name 93-K100861-0022-111-B05C-IH61
GenBank Accession LN515234 [GenBank]
Sequencing primer gene specific primer, IH61: 5'-GAACTTGTTGTGATGTTGCAGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K100861-0022-111-B05C-IH61
GCGTGATTGAGCGGTTATGACATTGCTCTGCCCCATCTTAGGCTGTCTTGCTACAATCTG
GCAGAATTGAAAGACCGCCACCGCGTTTCCACATATAAAATCTACAGTGCCGGTAATAAA
GCACTCCCTATAAAACTGGCGGTCACTATGAGGATACAAGGCATCCTGATATCCCTCGAC
ACGACATCTATAAATGACGGACATATCACCACTCACACGGAGGGCAACAGCTGGTCCTTT
TGCCGGTCCGGCCGTGTTTCGAAAGCACATGTCTACTCCGATGAATCCATTACCATTAGA
AGCTTCAAACATATTTTTAGAATAATTAGTAATGTCTTTAAGAATGTTATAGTTTGGTAT
AAAGAAGAGAAAACTATAAACTATTCAAGAAGAGAATACAAATTCTTACCAACAGTTGCG
GTGTTGTACGTTCTTCTTAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTC
Primers used for PCR IH62 - 3144, show primer details
   
Sequence Name 94-K100855-0022-111-B05C-3144
GenBank Accession LN515287 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K100855-0022-111-B05C-3144
CCTTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCGTTTCCCGCCTTCGGTTAAATTGCCTGTGATGATGGTTAAATCTT
GACCATCACCTATAAGAGTTAAATTGGGTTTCGTATTCTCAATGACGACGATTTCATCAT
AAATGCCTGTCTTTATATAGATGACGAATCTCTTTTGGCTGTGTTGAGGTGCCGCTGCAA
TGGCCGCATTCACTGTGCTGTACTTTCCGGTCCCATCCTTCGCGACCACAACATCAGCAA
TCTTCTTTAGAGCCTGCTTTATAAGAAACAGAAGAAATTCTATAAGCAGTAGAAAGAAAG
AAAAAAAAGGATAAAAGGTTTGTTAAGAGGGTCAAACATATCGATCGAATTGTGTTCTTC
ATTACGTGACCTTAAGGTCTATTTTTATACAAGGGCTGATCTCT
   
Sequence Name 94-K100861-0022-111-B05C-IH62
GenBank Accession LN515288 [GenBank]
Sequencing primer gene specific primer, IH62: 5'-ATTCCTTTTCCTTAGAGATCAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K100861-0022-111-B05C-IH62
GTATGAAGAACCAATTCGATCGATATGTTTGACCCTCTTAACAAACCTTTTATCCTTTTT
TTTCTTTCTTTCTACTGCTTATAGAATTTCTTCTGTTTCTTATAAAGCAGGCTCTAAAGA
AGATTGCTGATGTTGTGGTCGCGAAGGATGGGACCGGAAAGTACAGCACAGTGAATGCGG
CCATTGCAGCGGCACCTCAACACAGCCAAAAGAGATTCGTCATCTATATAAAGACAGGCA
TTTATGATGAAATCGTCGTCATTGAGAATACGAAACCCAATTTAACTCTTATAGGTGATG
GTCAAGATTTAACCATCATCACAGGCAATTTAACCGAAGGCGGGAAACGACAATCTGATC
CCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGC
GAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATC

Last Updated on 06.12.2018 16:11