Sequences from confirmation amplicon


Confirmation sequences for line 111D02 and hit At5g41310 are shown.
Line ID 111D02
Locus At5g41310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16519913
Primers used for PCR AK46 - 3144, show primer details
   
Sequence Name 01-K105916-0022-111-D02C-AK46
GenBank Accession LN486578 [GenBank]
Sequencing primer gene specific primer, AK46: 5'-TGTTTGTTTCTTGTTTTGTCCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K105916-0022-111-D02C-AK46
TTCCCATTTGCAGCGAATAAAAAAGTACACAAGAAGCAAATCTTACCGTCAAAAAAGCTA
TAAGGCTCAAATAAATGAAGTAGAGTTAGGGCAGTCTTTTAAACTGGTTTTTGGATGTTT
TTTGTTTGCAAATATGTGAAATCAATTTATTTCAAAATGAAATAAAAATATATGCTAATA
AGTTTTCATTGACGATTTTTTGTCATGAACAAATTGGTTTAATAATTTGATATAATATTT
TAATGAAATAATCATCACATTTATTTTTCAAAAGGTTTAGTTTTTAGATGCAAGCATTTG
AGGTTTATAATATATAAATTTTAATATTTGTTTTCAAAAGAAATATTTTCTAATAACAAA
AAAAAGCTAGGTTCTTCGAGGTATAGTGCCCCAACAATTATTTTTGTAATTATAGAAGTT
CAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGT
CCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGA
TTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCC
   
Sequence Name 01-K105917-0022-111-D02C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K105917-0022-111-D02C-3144
AAACTTTTCTGATTGTTAATCTCTCTATCAAGCTTTCGAGGACTAAGCGGATTGTTGATG
ACTTTCTCAGCTCCAGAGTGAGCATCCATACAGTCGAGCGAACGATGTCAGATTAGTTCA
TCACTTTTTAAAAGACA

Last Updated on 06.12.2018 16:11