Sequences from confirmation amplicon


Confirmation sequences for line 112B10 and hit At3g12320 are shown.
Line ID 112B10
Locus At3g12320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3925011
Primers used for PCR 07sf - 8474, show primer details
   
Sequence Name 29-K116518-0022-112-B10C-07sf
GenBank Accession LN499445 [GenBank]
Sequencing primer gene specific primer, 07sf: 5'-CTTTTTGTTTCTGGTTTGAAGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K116518-0022-112-B10C-07sf
TGTTAGATTCAACTATGGGGATGAACTGTCTTCTGAAGAATTCGTGTTGCAGGATCTACA
AAGGGCTTCACAAAAGGTCAGTTACCGATGATTTAGCTTGTAAACTTGCTTCGCATCCTA
GATTTTGTCTGATAGAATCTTGTCTTTGCTTTCTAGTTGACTGATGAGACACGCAAATGT
TTCCGGGATACTTTTTACCGACTTGCAAGGAGCTCACAAGATAAATCTGATTCAGTCAGC
CCCAACTCGGAAGAGCTGCTTATGCAAACGTCTAGATATGATTATGGTGATGGAAACAGG
TACATATCATATCTACTGCTGCGCACAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCACAGTTATTATAAT
   
Sequence Name 03-K116517-0022-112-B10C-8409
GenBank Accession LN487644 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K116517-0022-112-B10C-8409
TATGATATGTACCTGTTTCATCACCATAATCATATCTAGACGTTTGCATAAGCAGCTCTT
CCGAGTTGGGGCTGACTGAATCAGATTTATCTTGTGAGCTCCTTGCAAGTCGGTAAAAAG
TATCCCGGAAACATTTGCGTGTCTCATCAGTCAACTAGAAAGCAAAGACAAGATTCTATC
AGACAAAATCTAGGATGCGAAGCAAGTTTACAAGCTAAATCATCGGTAACTGACCTTTTG
TGAAGCCCTTTGTAGATCCTGCAACACGAATTCTTCAGAAGACAGTTCATCCCCATAGTT
TGAATCTAACATGTTCTCTTCAGACAGATCCGAGATTGTAAATGGAGCTTCCTCCTTCAA
ACCAAAAACAAAAAGA

Last Updated on 06.12.2018 16:11