Sequences from confirmation amplicon


Confirmation sequences for line 113E07 and hit At3g55260 are shown.
Line ID 113E07
Locus At3g55260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20490307
Primers used for PCR 06ja - 8474, show primer details
   
Sequence Name 30-K112831-0022-113-E07C-06ja
GenBank Accession LN499794 [GenBank]
Sequencing primer gene specific primer, 06ja: 5'-TTCTTGTGGTTTCAGGCTAATACA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K112831-0022-113-E07C-06ja
ACTTATAGTATAGTTTGAGACTATGTTGAGTGTTAATTTGATCATGATTCGTGAGCCTTG
AATTGTTTCATCGAGTTGTAGTTCACGAGTGTTATGGAATCTTTGCTAATTTGTGTTGTT
TCTTAAATTTGTTTGCAGACATTTAGCCAGTTGTGTGCCTTTGATTATATAACCAAATCT
GTGCAAATATATAAAGCACCATGGTATATTCAAGATAAACCTAGATTTGGATACCGCGGT
CTGCTGATAGGTAATAAGTCCGTATCTCTTTCTCTCATTTGCTTTCTTGTCCATCACATG
GAAAGAGAATGTAATCATGTGCTGGCTATTACTTACTACTTGTTCCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATT
   
Sequence Name 01-K112829-0022-113-E07C-8409
GenBank Accession LN486647 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K112829-0022-113-E07C-8409
AGTAATAGCCACACATGATTACATTCTCTTTCCATGTGATGGACACGATAGCAAATGAGA
GAAAGAGATACGGACTTATTACCTATCAGCATACCGCGGTATCCAAATCTAGGTTTATCT
TGAATATACCATGGTGCTTTATATATTTGCACAGATTTGGTTATATAATCAAAGGCACAC
AACTGGCTAAATGTCTGCAAACAAATTTAAGAAACAACACAAATTAGCAAAGATTCCATA
ACACTCGTGAACTACAACTCGATGAAACAATTCAAGGCTCACGAATCATGATCAAATTAA
CACTCAACATAGTCTTCAAACTATACTATTAAGTTACACACCTCCAATCCTCTAAGTGCA
CCATAAACTGTATTAGCCTGAAACCACAAGA

Last Updated on 06.12.2018 16:11