Sequences from confirmation amplicon


Confirmation sequences for line 113H08 and hit At4g25010 are shown.
Line ID 113H08
Locus At4g25010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12856394
Primers used for PCR KC63 - 8474, show primer details
   
Sequence Name 01-K035533-022-113-H08C-8409
GenBank Accession LN486478 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K035533-022-113-H08C-8409
GAGCCATTTACATGGACGTGGAGCTTGCATGTTGGTTTATATAAAGAAGGAAATAAGATT
AGAAAGATACTGAAGAGCTCGCTCATTCACAAAATAACCAAAATGGTTCTCACTCACAAC
GTATTGGCAGTTACATTTGGAGTCCTGGGTAAGTTTCAAGCATTCATAAATAAACATCAA
GTTATACAAAACTATATACTATATCATTGTTCTTATAAGTGCATATCAAATTTAACTTTG
TTATCTTTACCTATATGTATTTTTGTAGGTAACATCATATCGTTCATCGTTTTCTTGGCA
CCAGTGTAAGCATCATCGATCTCAAACGTACACTTCTATTTTTATTTGCTTTCTCTTAAC
AAAGAAAAATGTTTTGGACATTATTTTCAGGCCAACTTTTGTAAGAATCTGCAAGAAAAA
ATCGATAGAAGGTTTTGAATCACTTCCCTACGTGTCAGCCCTCTTTAGCGCGATGCTATG
GATTTACTATGCTCTGCAAAAAGATGGAGCAGGCTTCCTTCTCATTACCATAAACGCTGT
GGGATGCTTCATCGAAACCATCTACATCATCCTCTTCATCACCTATGCTAACAAGAAAGC
TAGAGTACGTTTAAACCATCCCATTTCTNTTGGTATATTAAAACATGCACTCATACATAC
AGGTNTAGCTGATGATGATNAACCATGTAGATATCAACTTTGAAGGTTCTTGGGCTCTTG
AATTTCTTGGGTTTTGCT
   
Sequence Name 49-K035533-022-113-H08C-KC63
GenBank Accession LN506755 [GenBank]
Sequencing primer gene specific primer, KC63: 5'-AGAATAATAGCAGCAAAACCCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K035533-022-113-H08C-KQ63
ATATCTACATGGTTTATCATCATCAGCTAAACCTGTATGTATGAGTGCATGTTTTAATAT
ACCAAAAGAAATGGGATGGTTTAAACGTACTCTAGCTTTCTTGTTAGCATAGGTGATGAA
GAGGATGATGTAGATGGTTTCGATGAAGCATCCCACAGCGTTTATGGTAATGAGAAGGAA
GCCTGCTCCATCTTTTTGCAGAGCATAGTAAATCCATAGCATCGCGCTAAAGAGGGCTGA
CACGTAGGGAAGTGATTCAAAACCTTCTATCGATTTTTTCTTGCAGATTCTTACAAAAGT
TGGCCTGAAAATAATGTCCAAAACATTTTTCTTTGTTAAGAGAAAGCAAATAAAAATAGA
AGTGTACGTTTGAGATCGATGATGCTTACACTGGTGCCAAGAAAACGATGAACGATATGA
TGTTACCTACAAAAATACATATAGGTAAAGATAACAAAGTTAAATTTGATATGCACTTAT
AAGAACAATGATATAGTATATAGTTTTGTATAACTTGATGTTTATTTATGAATGCTTGAA
ACTTACCCAGGACTCCNAATGTAACTGCCAATACGTTGTGAGTGAGAACCATTTTGGTTA
TTTTGTGAATGAGCGAGCTCTTCAGTATCTTTCTAATCTTATTTCCTTCTTTATATAAAC
CAACATGCTAGCTCCACGTCCATGTAAATGGCTTCATGTCCCGGGAATCTACATGGATCA
GCAATGAGTATGATGGGTCATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGT

Last Updated on 06.12.2018 16:11