Sequences from confirmation amplicon


Confirmation sequences for line 114C03 and hit At5g58430 are shown.
Line ID 114C03
Locus At5g58430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23621795
Primers used for PCR DN35 - 8474, show primer details
   
Sequence Name 11-K101828-0022-114-C03C-8409
GenBank Accession LN491053 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K101828-0022-114-C03C-8409
AATAAGATGCTGGGGTTGCTGAAGGTGGATAATACTGCAGCAGGCATGAACGGTTTGGGG
AAGACCATGAAGGAGAAGCTGAAGCAGTTCAATATTCAGTTTGATGAGATATGTAAAGTG
CATTCGACGTGGGTAGTGTTTGACGAGCAGCTGAAAGAAGAGCTAAAGATCTCACTGGCC
AGGCTTTTGGTACCGGCATACGGGAGCTTCATCGGCAGGTTCCAAAACCTTGGAGACATA
GGGAAAAACGCAGACAAGTACATCAAGTATGGAGTTGAGGACATAGAGGCGCGTATTAAC
GAGCTGTTCAAAGGGACTACCACGGGAAGAAAATGAAGGTAACGAACACAAAAATAGCCT
CTTGTAACATCTTCGTAACGAACCACCTGCTAAATGACCAATAGCGAAGTCTTGCAGGGA
GGATTTGAAACATGTA
   
Sequence Name 55-K101831-0022-114-C03C-DN35
GenBank Accession LN508765 [GenBank]
Sequencing primer gene specific primer, DN35: 5'-ATACATGTTACAAATCCTCCCTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K101831-0022-114-C03C-DN35
GATGTTACAGAGGCTATTTTTGTGTTCGTTACCTTCATTTTCTTCCCGTGGTAGTCCCTT
TGAACAGCTCGTTAATACGCGCCTCTATGTCCTCAACTCCATACTTGATGTACTTGTCTG
CGTTTTTCCCTATGTCTCCAAGGTTTTGGAACCTGCCGATGAAGCTCCCGTATGCCGGTA
CCAAAAGCCTGGCCAGTGAGATCTTTAGCTCTTCTTTCAGCTGCTCGTCAAACACTACCC
ACGTCGAATGCACTTTACATATCTCATCAAACTGAATATTGAACTGCTTCAGCTTCTCCT
TCATGGTCTTCCCCAAACCGTTCATGCCTGCTGCAGTATTATCCACCTTCAGCAACCCCA
GCATCTTATTCCATGAGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAA
TGTCAGCACGGTTATT

Last Updated on 06.12.2018 16:11