Sequences from confirmation amplicon


Confirmation sequences for line 114E03 and hit At5g10600 are shown.
Line ID 114E03
Locus At5g10600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3351824
Primers used for PCR MS65 - 3144, show primer details
   
Sequence Name 39-K017405-022-114-E03C-MS65
GenBank Accession LN503182 [GenBank]
Sequencing primer gene specific primer, MS65: 5'-GAATATCATCAATGAGTCGCTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K017405-022-114-E03C-ms65
ATCTCTCATCCTCTGTATCTCAATCACTCTGTTCTCAAGACCTTTGTACCCAATCCATCT
CAACACTGGGAAATAGTCACAGACATTCATCGACGATAGGCTCGAGAAAAACCTTGATTT
GAATTCATCTAAGAACTTCGTCTCTGATTCTGAATCACTCTCATTGACCCCGCGATTTCC
AGATACTAATCTAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAA
TGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCA
TCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACG
TCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCATCTTTG
   
Sequence Name 43-K017405-022-114-E03C-3144
GenBank Accession LN504668 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K017405-022-114-E03C-3144
AGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCAT
TTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTAGATTAGT
ATCTGGAAATCGCGGGGTCAATGAGAGTGATTCAGAATCAGAGACGAAGTTCTTAGATGA
ATTCAAATCAAGGTTTTTCTCGAGCCTATCGTCGATGAATGTCTGTGACTATTTCCCAGT
GTTGAGATGGATTGGGTACAAAGGTCTTGAGAACAGAGTGATTGAGATACAGAGGATGAG
AGATGAGTATCTTCAGCGACTCATTGATGATATT
Primers used for PCR FL02 - 8474, show primer details
   
Sequence Name 39-K028350-022-114-E03C-8409
GenBank Accession LN503237 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K028350-022-114-E03C-8409
TATCCGAATCCGGCGTTGAACATTAACACTACAATTCCTTTGCATACATCATCAGAGTAA
AATTCAGGTTCTGATTCTTGAAGCTTCAAGAATTTCTCCACTACTGAACCAGTGGAATCA
AACTTCTTCATTCGAATATCATCAATGAGTCGCTGAAGATACTCATCTCTCATCCTCTGT
ATCTCAATCACTCTGTTCTCAAGACCTTTGTACCCAATCCATCTCAACACTGGGAAATAG
TCACAGACATTCATCGACGATAGGCTCGAGAAAAACCTTGATTTGAATTCATCTAAGAAC
TTCGTCTCTGATTCTGAATCACTCTCATTGACCCCGCGATTTCCAGATACTAATCTAAGC
ATGACGTGAGCGGTGAGAAGAGTGAATTGATACCTGAGATCCACTTTACAAGAATTACCA
CCGGAGAATCTGAAGAGACTCGAACAGAGATTGGAGACTTCTTCAGTTCGTATGGAAGAG
TTCTTCTGTAAGCTGACGGAGGAGAAGACTTCGAGAGTGGAGAGACGACGGAGAGTCCGC
CAGAGATCGCCATAAGGAGCAAATCCAAAGTTTTTGTAGCCGTAGCTGAAGTGATCGGAG
GTAATGGTCTTGGGACGATTCGCAAGGGCTATGTCGTGATTGGTGAAGCATTCTTCGATG
GAATCTGGAGAAGAGAGGATCAAAACTTCACGGCATCCGAATTTGAGGAACAGAACCGGG
CCGTAGTTTGAAGAGAGATGATGTAGAGCTTGTGGTAAAGGATACTTATTGATGAGATGA
AGATGACCGATGATCGGAAGTGGAGTAGGACTTGGAGGTAGTTTAGGGTTTTGACTCCAT
AGGAACTTGGTGATGAAGAAGGTCAAAGTAACTGTGAGCAAGAAGATGACCCAGAGATTT
TCCATTT
   
Sequence Name 39-K028350-022-114-E03C-FL02
GenBank Accession LN503238 [GenBank]
Sequencing primer gene specific primer, FL02: 5'-ACGAGATTGACTCAAAAAGGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K028350-022-114-E03C-FL02
CTTCTTGCTCACAGCTACTTTGACCTTCTTCATCACCAAGTTCCTATGGAGTCAAAACCC
TAAACTACCTCCAAGTCCTACTCCACTTCCGATCATCGGTCATCTTCATCTCATCAATAA
GTATCCTTTACCACAAGCTCTACATCATCTCTCTTCAAACTACGGCCCGGTTCTGTTCCT
CAAATTCGGATGCCGTGAAGTTTTGATCCTCTCTTCTCCAGATTCCATCGAAGAATGCTT
CACCAATCACGACATAGCCCTTGCGAATCGTCCCAAGACCATTACCTCCGATCACTTCAG
CTACGGCTACAAAAACTTTGGATTTGCTCCTTATGGCGATCTCTGGCGGACTCTCCGTCG
TCTCTCCACTCTCGAAGTCTTCTCCTCCGTCAGCTTACAGAAGAACTCTTCCATACGAAC
TGAAGAAGTCTCCAATCTCTGTTCGAGTCTCTTCAGATTCTCCGGTGGTAATTCTTGTAA
AGTGGATCTCAGGTATCAATTCACTCTTCTCACCGCTCACGTCATGCTTAGATTAGTATC
TGGAAATCGCGGGGTCAATGAGAGTGATTCAGAATCAGAGACGAAGTTCTTAGATGAATT
CAAATCAAGGTTTTTCTCGAGCCTATCGTCGATGAATGTCTGTGACTATTTCCCAGTGTT
GAGATGGATTGGGTACAAAGGTCTTGAGAACAGAGTGATTGAGATACAGAGGATGAGAGA
TGAGTATCTTCAGCGACTCATTGATGATATTCGAATGAAGAAGTTTGATTCCACTGGTTC
AGTAGTGGAGAAATTCTTGAAGCTTCAAGAATCAGAACCTGAATTTTACTCTGATGATGT
TATNCAAGGAAATTGTAGTGTA
Primers used for PCR 22wp - 3144, show primer details
   
Sequence Name 27-K118496-0022-114-E03C-22wp
GenBank Accession LN498604 [GenBank]
Sequencing primer gene specific primer, 22wp: 5'-AAAGGAAGAAAAATGAAGCACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K118496-0022-114-E03C-22wp
TCGCCTTATTATCAATATTTATATGTAAAAACCATCCTCTAAATCAACTCTCTTGGCCAA
CACAGAGGAGACTAGAGCTGAGACAAGATAGGAACCATTTCTGGGCATGGCTTAAGCAAT
GCAATAAGCGGCTCAGCCCTATCCATTGCCACACTGAACGCAGGCCTCATGTCTATGTCT
TCTTTTTCTACCTTCTCCCACTCAAAACATTGAACCAATGCTCCAACAGCAAGCGACACT
ACCCTCATTGCAAGTCCAGCTGCAGGGCACGCCCGCCTCCCAACTCCAAATGGCAAAAAC
CTAAAACCATCTCTATCGCCAACAAACTCTTCAAATCTCTCCGGCTTGAAAACATCTGCT
TCTTCCCAAAGCTCACCGTCCCTATGAACAGCCCAAGCATTCACCAACAACACTGTATTT
TCAGGGATCTCGTAGTTGCCTAAGTTGAATCTCTTGGATGAACAGTGAGGAGGCAAGATA
GGAGCTGCAGGGTGTAGCCTAAGCGTCTCATAGATGACACATCGAAGATAAGGTAAGCTT
GAGAGATCCGAGTCTTGTATAATCCCTTTGTGTTTAACATTAGATTTGATTTCTTCTCTG
ACCTTGTCAAGCTTATCTGGATGGTTCAGTAAAAGCGCCATTGCCCATTCCATTGTCACC
AATGAACATTAAATTGTAAACAATTCCGGCGTTGAACATTAACACTACAATTCCTTTGAT
AACATCATCAGAGTAAAATTCAGGTTCTGATTCTTGAAGCTTCAAGAATTTCTCCACTAC
TGAACCAGTGGAATCAAACTTCTTCATTCGAATATCATCAATGAGTCGCTGAAGATACTC
ATCTCTCATCCTCTGTATCTCAATCACTCTGTTCTCAAGACCTTTGTACCCCATCCATCT
CAACACTGGGAAATAGTCACAGACATTCAT
   
Sequence Name 03-K118495-0022-114-E03C-35St
GenBank Accession LN487686 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K118495-0022-114-E03C-35St
AAGGAAGTTCATTTCATTTGGAGAGGTAAGGGTACCCGGGGATCAGATTGTCGTTTCCCG
CCTTAGATTAGTATCTGGAAATCGCGGGGTCAATGAGAGTGATTCAGAATCAGAGACGAA
GTTCTTAGATGAATTCAAATCAAGGTTTTTCTCGAGCCTATCGTCGATGAATGTCTGTGA
CTATTTCCCAGTGTTGAGATGGATTGGGTACAAAGGTCTTGAGAACAGAGTGATTGAGAT
ACAGAGGATGAGAGATGAGTATCTTCAGCGACTCATTGATGATATTCGAATGAAGAAGTT
TGATTCCACTGGTTCAGTAGTGGAGAAATTCTTGAAGCTTCAAGAATCAGAACCTGAATT
TTACTCTGATGATGTTATCAAAGGAATTGTAGTGTTAATGTTCAACGCCGGAATTGTTTA
CAATTTAATGTTCATTGGTGACAATGGAATGGGCAATGGCGCTTTTACTGAACCATCCAG
ATAAGCTTGACAAGGTCAGAGAAGAAATCAAATCTAATGTTAAACACAAAGGGATTATAC
AAGACTCGGATCTCTCAAGCTTACCTTATCTTCGATGTGTCATCTATGAGACGCTTAGGC
TACACCCTGCAGCTCCTATCTTGCCTCCTCACTGTTCATCCAAGAGATTCAACTTAGGCA
ACTACGAGATCCCTGAAAATACAGTGTTGTTGGTGAATGCTTGGGCTGTTCATAGGGACG
GTGAGCTTTGGGAAGAAGCAGATGTTTTCAAGCCGGAGAGATTTGAAGAGTTTGTTGGCG
ATAGAGATGGTTTTAGGTTTTTGCCATTTGGAGTTGGGAGGCGGGCGTGCCCTGCAGCTG
GACTTGCCATGAGGTTAGTGTCGCTTGCTGTTGGAGCATTGGTTCATGTTTTGAGTGGGA
GAGGTAGAAA

Last Updated on 06.12.2018 16:11