Sequences from confirmation amplicon


Confirmation sequences for line 114H11 and hit At1g15170 are shown.
Line ID 114H11
Locus At1g15170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5221582
Primers used for PCR BM05 - 8474, show primer details
   
Sequence Name 15-K015833-022-114-H11C-8409
GenBank Accession LN492741 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K015833-022-114-H11C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCACATGTAAATG
ACTGATCCATAGCCGCAGAAGGAAGAGCATATTTGAAGAACTCTCCAATGCCATCGAATA
TCTCCATGGAGAGCGGCGCACGTGTCTCAGAACAGGCAGAGGAGTAGTACATGAAAGATC
CAAGGAAAATGGCATAGAGCCAGTTTGACAAACTGATAGCCAAAGCTCCTCCAAGATTAC
CAAGCCCTGAGTTGTAAACCAAAAACCAGCAGAGAGGAACGTGGATACAGAACACAACAT
AGGAGGTGATGAGGAGAGGTGTGATCAAACTCTGGTTTTGAAAGTAGCGAGTGAGCGGCT
GTAGAACAGCGTAAGCAAACAGTCCTGGGATAAGCCAGGTGGCATATTTTCCGGCTTCGT
GAGCAATAGAAGGGTCTTGGCCAAGGATCAAAAGAAGCTTTTCCATGTTGAACCAAATGA
GAGAAAGAGGGAGACACACTAATGCAAGACAGAACATAGCAGTGTAAGTCTGAACTCCTA
GTTTCCGGTATAG
   
Sequence Name 16-K015833-022-114-H11C-BM05
GenBank Accession LN493205 [GenBank]
Sequencing primer gene specific primer, BM05: 5'-TATGGAGCTAAGCTATACCGGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K015833-022-114-H11C-bm05
TTCAGACTTACACTGCTATGTTCTGTCTTGCATTAGTGTGTCTCCCTCTTTCTCTCATTT
GGTTCAACATGGAAAAGCTTCTTTTGATCCTTGGCCAAGACCCTTCTATTGCTCACGAAG
CCGGAAAATATGCCACCTGGCTTATCCCAGGACTGTTTGCTTACGCTGTTCTACAGCCGC
TCACTCGCTACTTTCAAAACCAGAGTTTGATCACACCTCTCCTCATCACCTCCTATGTTG
TGTTCTGTATCCACGTTCCTCTCTGCTGGTTTTTGGTTTACAACTCAGGGCTTGGTAATC
TTGGAGGAGCTTTGGCTATCAGTTTGTCAAACTGGCTCTATGCCATTTTCCTTGGATCTT
TCATGTACTACTCCTCTGCCTGTTCTGAGACACGTGCGCCGCTCTCCATGGAGATATTCG
ATGGCATTGGAGAGTTCTTCAAATATGCTCTTCCTTCTGCGGCTATGGATCAGTCATTTA
CATGTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCT

Last Updated on 06.12.2018 16:11