Sequences from confirmation amplicon


Confirmation sequences for line 116B05 and hit At2g14440 are shown.
Line ID 116B05
Locus At2g14440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:6146221
Primers used for PCR NR76 - 8474, show primer details
   
Sequence Name 70-K102506-0022-116-B05C-8409
GenBank Accession LN512360 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K102506-0022-116-B05C-8409
TTACAATTGAATATATCCTGAGCAACAGAAGTACTTATTTACACTAAAATCTTCCACTTA
ATTTTTGGACAATCGTGAGCTCATGAATTATACCATATTGTTGATATATTGTTAGGTCGA
ACTACTTCTAAGAGTTCACCACGTGAATTTAGTAAGCCTTGTTGGATACTGTGATAAAGG
AAATGACTTGGCTCTCATCTACGAGTTCATGGAAAATGGAAACTTAAAGGAACATCTCTC
GGGTAAAAAGTTAGAGTATGATGCAAAGTTTTTTCAGTTACATGCCAAAGGCTTTAAAAA
TTTGTATTGATTTCTATCCATGTTGTGAATTTTGGCAGGAAAACGTGGTGGCCCTGTCTT
GAATTGGCCTGGTAGACTGAAAATAGCTATTGAGTCTGCACTAGGTTTGTCGAAATTTCA
TTAATTCCTTGTTATATTACTTTTTCTAAAGTTTATATATATTCTTTTTCCTTCTTCCTT
TAAACTATCTTCATAAATAGTTAACATATGAAGATTTTTCTGTGTACTAGGAATTGAGTA
CTTGCATATTGGATGTAAGCCGCCAATGGTTCACAGAGATGTAAAAAGTACCAATATACT
GTTAGGTCTACGGTTTGAAGCCAAACTTGCCGATNTCGGACTTTCAAGATCTTT
   
Sequence Name 70-K102507-0022-116-B05C-NR76
GenBank Accession LN512361 [GenBank]
Sequencing primer gene specific primer, NR76: 5'-TTCCCACTAGGAAAGATCTTGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K102507-0022-116-B05C-NR76
CTTCACCGTAGACCTAAAGTATATTGGTACTTTTTACATCTCTGTGAACCATTGGCGGCT
TACATCCAATATGCAAGTACTCAATTCCTAGTACACAGAAAAATCTTCATATGTTAACTA
TTTATGAAGATAGTTTAAAGGAAGAAGGAAAAAGAATATATATAAACTTTAGAAAAAGTA
ATATAACAAGGAATTAATGAAATTTCGACAAACCTAGTGCAGACTCAATAGCTATTTTCA
GTCTACCAGGCCAATTCAAGACAGGGCCACCACGTTTTCCTGCCAAAATTCACAACATGG
ATAGAAATCAATACAAATTTTTAAAGCCTTTGGCATGTAACTGAAAAAACTTTGCATCAT
ACTCTAACTTTTTACCCGAGAGATGTTCCTTTAAGTTTCCATTTTCCATGAACTCGTAGA
TGAGAGCCAAGTCATTTCCTTTATCACAGTATCCAACAAGGCTTACTAAATTCACGTGGT
GAACTCTTAGAAGTAGTTCGACCTAACAATATATCAACAATATGGTATAATTCATGAGCT
CACGATTGTCCAAAAATTAAGTGGAAGATTTTAGTGTAAATAAGTACTTCTGTTTGCTCA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATT
TCGACGTGTCTA

Last Updated on 06.12.2018 16:11