Sequences from confirmation amplicon


Confirmation sequences for line 117C02 and hit At1g69380 are shown.
Line ID 117C02
Locus At1g69380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26081860
Primers used for PCR KM76 - 8474, show primer details
   
Sequence Name 13-K101217-0022-117-C02C-8409
GenBank Accession LN491963 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K101217-0022-117-C02C-8409
GCCATTTCCTCCAGAGGTGATGCAGAACCGACAATCAGATCTTTTAGAATGGTGCATTAT
CTTTTTACTGGCCATTGAAAACGCAATAGGCATTTACGAGATTGTTCGAGAATCCGCAGG
AGCTTCACTCTGATGCTACAAAAGTATAAATTCATTGTAGTGAACTCAAATTCAAGTTCA
TTTGTTTAGGTCCTTCTGTAATAAAATCAACTTGACTTGCTAGCTTGCGCAGCTTATGTC
AACATTATGGTTTTCGGAAAAATAGGATCAATACAAATGTTATGCTTCCTTCTTTGAAAA
CATTAATCCAGTCTTCTAAGCAAGTACTCAATTTGGACTTCCTTTGTGAGGGGCATGATC
CTTTCTCCATAGAGTTTTGCAA
   
Sequence Name 13-K101223-0022-117-C02C-KM76
GenBank Accession LN491964 [GenBank]
Sequencing primer gene specific primer, KM76: 5'-GTTGCAAAACTCTATGGAGAAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K101223-0022-117-C02C-KM76
AATTGAGTACTTGCTTAGAAGACTGGATTAATGTTTTCAAGAAGGAAGCATAACATTTGT
ATTGATCCTATTTTTCCGAAAACCATAATGTTGACATAAGCTGCGCAAGCTAGCAAGTCA
AGTTGATTTTATTACAGAAGGACCTAAACAAATGAACTTGAATTTGAGTTCACTACAATG
AATTTATACTTTTGTAGCATCAGAGTGAAGCTCCTGCGGATTCTCGAACAATCTCGTAAA
TGCCTATTGCGTTTTCAATGGCCAGTAAAAAGATAATGCACCATTCTAAAAGATCTGATT
GTCGGTTCTGCATCACCTCTTGGAGGAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCAACA
Primers used for PCR KM77 - 3144, show primer details
   
Sequence Name 14-K101217-0022-117-C02C-3144
GenBank Accession LN492433 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K101217-0022-117-C02C-3144
TCCACTATCCTTCGCAAGACCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTAAAAAA
CTCAAAAACATGATTACAGTTAATGATAACAAAATCAACCTAGTAATGTAATAATAATAC
CTCTATAAACTTTAGCTTATAGTCTAGATCCCCAAATCTCTGAGAGATCTCGTACTCTTC
TCTTAGGTACTCGTATATCTGAGCATATCTNGCTTCTCTCCAAGCAATCTCGGATCTATG
AGAAAACAAGAGAACATATGAGTCTCAGCATATATATATAGAGATCATAGAAAACAACAA
TATAAAGTGATTTAACATTAGTAGAATACCTCTCAAACAAACCAACTTGGAGAATC
   
Sequence Name 14-K101223-0022-117-C02C-KM77
GenBank Accession LN492434 [GenBank]
Sequencing primer gene specific primer, KM77: 5'-TGATTCTCAAAGTTGGTTTGTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K101223-0022-117-C02C-KM77
TTTATTTGTTGTTTTCATGATCTCTATTATATATGCTGAGACTCATATGTTCTCTTGTTT
TCTCATAGATCCGAGATTGCTTGGAGAGAAGCTAGATATGCTCAGATATACGAGTACCTA
AGAGAAGAGTACGAGATCTCTCAGAGATTTGGGGATCTAGACTATAAGCTAAAGTTTATA
GAGGTATTATTATTACATTACTAGGTTGATTTTGTTATCATTAACTGTAATCATGTTTTT
GAGTTTTTTACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTA
CCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATA
GTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATCTC

Last Updated on 06.12.2018 16:11