Sequences from confirmation amplicon


Confirmation sequences for line 117E05 and hit At1g30860 are shown.
Line ID 117E05
Locus At1g30860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10987363
Primers used for PCR 04vh - 8474, show primer details
   
Sequence Name 45-K117914-0022-117-E05C-04vh
GenBank Accession LN505673 [GenBank]
Sequencing primer gene specific primer, 04vh: 5'-TCAGTGTCTTGAGAACTCCATGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K117914-0022-117-E05C-04vh
GCTCAGTCATCTCTATCATCTTGCTCTTCACCTCCTCTTAATGGCTCTTCTTTGATTTCT
TCATCAATTTCAAAATTTACTTCCTCCTCTTCTTCTTGTTGCAATTCCCATTTTTTGGAG
TGCTTATCCGAGTGTGTCTGTACACGGGACATCTCATGATAGAGGATGTCATGCATGAAG
CCATTTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATCTC
   
Sequence Name 05-K117913-0022-117-E05C-8409
GenBank Accession LN488540 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117913-0022-117-E05C-8409
ATCCTGGCAATGGCTTCATGCATGACATCCTCTATCATGAGATGTCCCGTGTACAGACAC
ACTCGGATAAGCACTCCAAAAAATGGGAATTGCAACAAGAAGAAGAGGAGGAAGTAAATT
TTGAAATTGATGAAGAAATCAAAGAAGAGCCATTAGGAGGAGGTGAAGAGCAAGATGATC
GAGATGACTTGAGCCAATCGTCGTCTTCACATATATCTGCATCGTCTCCTGCGGGATCAT
GGAGTTCTCAAGAC

Last Updated on 06.12.2018 16:11