Sequences from confirmation amplicon


Confirmation sequences for line 117F08 and hit At1g04990 are shown.
Line ID 117F08
Locus At1g04990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1421013
Primers used for PCR 10ht - 8474, show primer details
   
Sequence Name 32-K112586-0022-117-F08C-10ht
GenBank Accession LN500648 [GenBank]
Sequencing primer gene specific primer, 10ht: 5'-TACCTCTGTTCTTCCTCGTCATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K112586-0022-117-F08C-10ht
CAAATCTGTTTCTTTCGATGATCATCGTGACACTTTCTCTGTGATTTTTCCTTTCTTCTG
TTGTTGTTGATCATCGTGATCATCGTCTTTCCTTACATGCGAACCCCCATGTCAGACACT
CAGCATGTACAGAGCTCTTTGGTGTCGATTCGATCTTCCGACAAAATCGAAGGTTAACTT
TCTTCTAATCTCGAGAGATCCATTTGATCCGGTCTGGTTTTTCCGTAATGTGTTTGAAAG
ATCATACTGAGTTTTCTAGTTTGATCATCGTTTATAACTCTCATTGTGATTTCAGCAAAT
TTTTGTAGCATTTCGGAATTTTGATCTCTTGATGAGTTTTATTTACGTTTTCTGTTTGCT
TTTTTTATCTAGTGATCAAGTTTTGAGATTTATGTTGTTGTTGTTATGATGATCTTGTAG
ATGCCTTTAGGAAGATGAAAGTAAATGAGACCGGCGTGGAGGAACTGAATCCGTATCCAG
ATCGTCCCGGTGAAAGAGATTGCCAATTCTATTTAAGAACTGGTCTCTGTGGCTATGGCA
GCGGCGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGC
   
Sequence Name 06-K112585-0022-117-F08C-8409
GenBank Accession LN488884 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K112585-0022-117-F08C-8409
CATTTACATTGAATATATCCTGCCGCCGCTGCCATAGCCACAGAGACCAGTTCTTAAATA
GAATTGGCAATCTCTTTCACCGGGACGATCTGGATACGGATTCAGTTCCTCCACGCCGGT
CTCATTTACTTTCATCTTCCTAAAGGCATCTACAAGATCATCATAACAACAACAACATAA
ATCTCAAAACTTGATCACTAGATAAAAAAAGCAAACAGAAAACGTAAATAAAACTCATCA
AGAGATCAAAATTCCGAAATGCTACAAAAATTTGCTGAAATCACAATGAGAGTTATAAAC
GATGATCAAACTAGAAAACTCAGTATGATCTTTCAAACACATTACGGAAAAACCAGACCG
GATCAAATGGATCTCTCGAGATTAGAAGAAAGTTAACCTTCGATTTTGTCGGAAGATCGA
ATCGACACCAAAGAGCTCTGTACATGCTGAGTGTCTGACATGGGGGTTCGCATGTAAGGA
AAGACGATGATCACGATGATCAACAACAACAGAAGAAAGGAAAAATCACAGAGAAAGTGT
CACGATGATCATCGAAAGAAACAGATTTTTGTTATCCTCTATTTTTCTGGGTAAGAAATG
ACGAGGAAGACNAGAGGTA

Last Updated on 06.12.2018 16:11