Sequences from confirmation amplicon


Confirmation sequences for line 119B02 and hit At3g49410 are shown.
Line ID 119B02
Locus At3g49410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18327009
Primers used for PCR 18ra - 8474, show primer details
   
Sequence Name 77-K115895-0022-119-B02C-18ra
GenBank Accession LN513595 [GenBank]
Sequencing primer gene specific primer, 18ra: 5'-TCTTATCTGGCGAGTAGAAAGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K115895-0022-119-B02C-18ra
AGATCCCAAGAAACTGAAGTGGGAAGACTTTGTTTCTCGCAGCTCCACCACTGGCAATGG
CAGGTAGCAGTGTCTGCATTGTTTGAGGAGCGGCCTATATGGACAAGGGATTCTGTTGTT
CAACGACTACTTGATAAAGGTCTTAAATGTACACATCATATGTTAAACAGGTGATATAGC
TTTCTAATACGGGGTTCTTATACTGAGGTGTTTTTAGTACTATATTGATCAATTTGGTCT
CTCTGCAGGTTTCTGCTCAGAGCTGCATATTATTTCTCCAGTGGTCCGTTTCTTAGGTTC
TGGATTAAAAGAGGCTATGATCCACGGAATGATCCTGAGTCTCGTGTGTAAGTTCTCTTT
TAAGACATTGATTTGTATATTCTTATTGTATGGTTTGTGACATTTTTTAGGTGAATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCACGGTTATT
   
Sequence Name 47-K115894-0022-119-B02C-8409
GenBank Accession LN506299 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K115894-0022-119-B02C-8409
TTACATTGAATTATTCACCTAAAAATGTCACAAACCATACAATAAGAATATACAAATCAA
TGTCTTAAAAGAGAACTTACACACGAGACTCAGGATCATTCCGTGGATCATAGCCTCTTT
TAATCCAGAACCTAAGAAACGGACCACTGGAGAAATAATATGCAGCTCTGAGCAGAAACC
TGCAGAGAGACCAAATTGATCAATATAGTACTAAAAACACCTCAGTATAAGAACCCCGTA
TTAGAAAGCTATATCACCTGTTTAACATATGATGTGTACATTTAAGACCTTTATCAAGTA
GTCGTTGAACAACAGAATCCCTTGTCCATATAGGCCGCTCCTCAAACAATGCAGACACTG
CTACCTGCCATTGCCAGTGGTTGGAGCTGCGAGAAACAAAGTCTTCCCACTTCAGTTTCT
TTGGGATCTGTATTGAAAGCTAAGGACAGACCAAGCAAATACAGCTCTTTCTACTCGCCA
GATAAG

Last Updated on 06.12.2018 16:11