Genotyping and primer details


Genotyping and primer details for 119B02 - At3g49410
Line ID 119B02
Locus At3g49410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18327009 of line 119B02 Chr3:18327009
   
Insertion allele amplicon 8474 - 18ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18ra: 5'-TCTTATCTGGCGAGTAGAAAGAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18326535
   
wt amplicon (over InsPos) 21rt - 18ra
wt amplicon start Chr3:18326535
wt amplicon end Chr3:18327569
wt amplicon size 1035 bp
Locus specific primer 18ra: 5'-TCTTATCTGGCGAGTAGAAAGAGC-3'
2nd locus specific primer 21rt: 5'-GCTTTAATTCAACAGGCAAAAGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11