Sequences from confirmation amplicon


Confirmation sequences for line 119D02 and hit At5g64710 are shown.
Line ID 119D02
Locus At5g64710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25871750
Primers used for PCR 09kh - 8474, show primer details
   
Sequence Name 33-K113531-0022-119-D02C-09kh
GenBank Accession LN501073 [GenBank]
Sequencing primer gene specific primer, 09kh: 5'-ACGGTTTTTACATAGAACCAATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K113531-0022-119-D02C-09kh
AACTATATAATAAACCGGAATCTTTAGCCTACCGTTGTTTGTTTCCCGGTTAAATTAAAC
ATCTTCAATCTTGAATCCTAGAGTCACCTGATGGTTTCTTCCTCAAGCTCATCAATATTC
CCTCAACAAGCTTATCTTCACCAAAAGTTTTTGCAGGCTTCTTATCAGTGTCCCAAGAAC
CCAGAATTTGAAGTAAATCACTCTCTGTTTCATCTTCTTTCTTCTTCCTTTCCCCACCGC
TCTGTTTTGAAGAAGCTTTTTCCTCCATCTCTGAGTCATAATCTTCTCCCTCTGAAACAG
ATGACTCATTCTCATGAATCTTTTTGGAAACTGGACCGAGATCTTCGAATGATGAATCAG
ATTCACATGGAACCGGGGTTGACGAAGTAACAATGTGCCCTGATTCACATGGACAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGACCGCA
   
Sequence Name 08-K113530-0022-119-D02C-8409
GenBank Accession LN489807 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K113530-0022-119-D02C-8409
TATATCCTGTCCATGTGAATCAGGGCACATTGTTACTTCGTCAACCCCGGTTCCATGTGA
ATCTGATTCATCATTCGAAGATCTCGGTCCAGTTTCCAAAAAGATTCATGAGAATGAGTC
ATCTGTTTCAGAGGGAGAAGATTATGACTCAGAGATGGAGGAAAAAGCTTCTTCAAAACA
GAGCGGTGGGGAAAGGAAGAAGAAAGAAGATGAAACAGAGAGTGATTTACTTCAAATTCT
GGGTTCTTGGGACACTGATAAGAAGCCTGCAAAAACTTTTGGTGAAGATAAGCTTGTTGA
GGGAATATTGATGAGCTTGAGGAAGAAACCATCAGGTGACTCTAGGATTCAAGATTGAAG
ATGTTTAATTTAACCGGGAAACAAACAACGGTAGGCTAAAGATTCCGGTTTATTATATTA
GTTTTTGGTGGAGCTTCGGTTTGGCGATTGGTTCTATGTAAAAACCG

Last Updated on 06.12.2018 16:11