Sequences from confirmation amplicon


Confirmation sequences for line 119F07 and hit At2g47850 are shown.
Line ID 119F07
Locus At2g47850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19596417
Primers used for PCR 08et - 8474, show primer details
   
Sequence Name 32-K110907-0022-119-F07C-08et
GenBank Accession LN500631 [GenBank]
Sequencing primer gene specific primer, 08et: 5'-CTGACTGTGCTCAATTTTGTGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K110907-0022-119-F07C-08et
CGACTCGTACCTGAGAGACCAGGTGCTCCTGACTGTGCCTACTACATGCGAACTGGTGTC
TGTGGCTACGGTAATAGATGTCGTTACAATCACCCTCGCGATCGTGCCTCGGTAATTTCT
TCCTCTCATTCATTCTTCTTTCTTATTATTGTTCTGAAACCATCCATACTAGGAGGACAC
AATCAAATGCTTCTCTCTGTTTTTTCCGCAATGTGGGTGATCACTGGCCTTATAGAGTCT
CTCTAAACTGGGGAAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAAGGTTATTA
   
Sequence Name 16-K110898-0022-119-F07C-8409
GenBank Accession LN493478 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K110898-0022-119-F07C-8409
TTTCCCCGTTTAGAGAGACTCTATAAGGCCAGTGATCACCCACATTGCGGAAAAAACAGA
GAGAAGCATTTGATTGTGTCCTCCTAGTATGGATGGTTTCAGAACAATAATAAGAAAGAA
GAATGAATGAGAGGAAGAAATTACCGAGGCACGATCGCGAGGGTGATTGTAACGACATCT
ATTACCGTAGCCACAGACACCAGTTCGCATGTAGTAGGCACAGTCAGGAGCACCTGGTCT
CTCAGGGTACGAGTCGCTACCCAACCCTAATCGCCACATCGATTCTGCAAATCACAAAAT
TGAGCACAGTCA

Last Updated on 06.12.2018 16:11