Sequences from confirmation amplicon


Confirmation sequences for line 119F07 and hit At2g47860 are shown.
Line ID 119F07
Locus At2g47860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19601393
Primers used for PCR 65hw - 3144, show primer details
   
Sequence Name 24-K112477-0022-119-F07C-65hw
GenBank Accession LN497057 [GenBank]
Sequencing primer gene specific primer, 65hw: 5'-CCACCTCTAGTCTCAGCTCTCTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K112477-0022-119-F07C-65hw
GCTCCGCTTGCCTCCCGCTTCCCGAGCGGTTTGGGAACAGAAGAACTGGCTTTGGTTCGA
ACCGCCTGTGCCACCGCCGCTGGTCATGGCGTTCTTGAGCCGTGTCTGTTCGTAGAACAG
GACTTGAACCGCAATCTGAACCGGAAGTCTCTCGTTTTGCGCCGCGTGGCTGCTTGCGTC
CTGCGACAGTTTCTTACAGCTGACTGTCTTGCAGAGACGGTAACGTTCCGAATCTTTCAT
GTGGGGGTGAACCTAATACAAAACCGTAACACAGCAATCAAAATCATACAACACTCATTC
TCAAAAGCAGACTCTTTTATACCTTGAGGAAGATATCAACGGCTCGGTAGAGGCCATCGC
AGACGACGCCNGGGAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCC
CCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGTGGAAGGGTCTTGCG
AAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATC
   
Sequence Name 01-K112476-0022-119-F07C-3144
GenBank Accession LN486638 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K112476-0022-119-F07C-3144
CAGACCCTTCCACTATATAAGGAAGTTCATTTCATTTGGAGAGGACTGGGTACCCGGGGA
TCAGATTGTCGTTTCCCGCCTTCGGTTTAACTATCAGTGTTTCCCTGGCGTCGTCTGCGA
TGGCCTCTACCGAGCCGTTGATATCTTCCTCAAGGTATAAAAGAGTCTGCTTTTGAGAAT
GAGTGTTGTATGATTTTGATTGCTGTGTTACGGTTTTGTATTAGGTTCACCCCCACATGA
AAGATTCGGAACGTTACCGTCTCTGCAAGACAGTCAGCTGTAAGAAACTGTCGCAGGACG
CAAGCAGCCACGCGGCGCANAACGAGAGACTTCCGGTTCAGATTGCGGTTCAAGTCCTGT
TCTACGAACAGACACGGCTCAAGAACGCCATGACCAGCGGCGGTGGCACAGGCGGTTCGA
ACCAAAGCCAGTTCTTCTTGTTCCCAAACCGCTNGGGAAGCGGGATGGCAAGCGGAGCCA
TCTCGCCAAGGGACAACTATGCATCAGTGAGAAGAGAGAACAGAGAGCTGAGACTAGAGG
TGG

Last Updated on 06.12.2018 16:11