Sequences from confirmation amplicon


Confirmation sequences for line 119F12 and hit At3g14070 are shown.
Line ID 119F12
Locus At3g14070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4661444
Primers used for PCR 05nt - 8474, show primer details
   
Sequence Name 69-K114626-0022-119-F12C-05nt
GenBank Accession LN512257 [GenBank]
Sequencing primer gene specific primer, 05nt: 5'-TCCAAGGAGATTAACAATTCCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K114626-0022-119-F12C-05nt
CGTCTCATTAGCGCCTGTCCTTTTGGCGTCTCTCTGGAGTAGCCAAGACGATGTGAGTCT
CCAAGCTTGTGGTGTCGCATACTTTTTCAGCGTTGTTATAGGCTCCACTCTTGGCTTTCT
GGCTTACAAAAACACAGAACCTGATCACCCACCTCGAAGATTCTTGATCCCTTGGGTTCT
TGGTGGGTTTATAATGAGCATCGTATGGTTCTACATGATAGCAAACGAACTCGTTGCTCT
TCTGGTGACATTTGGTGAGATTTACGGAATCAACCCGTCGATACTTGCGCTAACAGTACT
TGCTTGGGGAAACTCAATGGGTGACTTAGTATCGAATATCGCATTGACAATGAACGGTGG
GGATGGTGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 69-K114625-0022-119-F12C-8409
GenBank Accession LN512256 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K114625-0022-119-F12C-8409
ATTTACAATTGAATATATCCTGACACCATCCCCACCGTTCATTGTCAATGCGATATTCGA
TACTAAGTCACCCATTGAGTTTCCCCAAGCAAGTACTGTTAGCGCAAGTATCGACGGGTT
GATTCCGTAAATCTCACCAAATGTCACCAGAAGAGCAACGAGTTCGTTTGCTATCATGTA
GAACCATACGATGCTCATTATAAACCCACCAAGAACCCAAGGGATCAAGAATCTTCGAGG
TGGGTGATCAGGTTCTGTGTTTTTGTAAGCCAGAAAGCCAAGAGTGGAGCCTATAACAAC
GCTGAAAAAGTATGCGACACCACAAGCTTGGAGACTCACATCGTCTTGGCTACTCCAGAG
AGACGCCAAAAGGACAGGCGCTAATGAGACGCTGGCCACAGCATATGTCTTTGACCATGT
ATCTTCTTCGACCGATGGAATTGTTATCTTCCTTGG

Last Updated on 06.12.2018 16:11