Sequences from confirmation amplicon


Confirmation sequences for line 120B03 and hit At3g03120 are shown.
Line ID 120B03
Locus At3g03120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:717125
Primers used for PCR 07lq - 8474, show primer details
   
Sequence Name 24-K114022-0022-120-B03C-07lq
GenBank Accession LN497079 [GenBank]
Sequencing primer gene specific primer, 07lq: 5'-ACTTTACGAGTCGAGAAACGATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K114022-0022-120-B03C-07lq
CTTATACGTCCAAGAATTTTCTGCTCAGGTATATAAGTAATTGCTACAGATTAGATTGGT
ATCTTCTTTTATACCACATTTCATCAAAAACTCTAATGAATGGATTATTTTTGTTCAGGA
TAACAAAGATTTATATGCTGAGGAAGCTGCTGCCCAAATGGAGAGAGAGAGACAAAGAAT
GCTTTCGATTCCTGGACTTATAGCTCCCAGCGAAATTCAAGACGACATGGCCGATTCTTA
AGTCTTGTTTTTATATTTTATAAGAAGAAGGCTCTCGAAAAGAGAAATGGAATTTTTTTT
GTAGAGATCTTGAGAAATGGAAAGCTTAGAAAAGACAAGTGTTAGGTTTTCTAAATTACA
ATCTTTTTTGTTGGATTTGTTACTATTAAATGAAAAATGTTTAGGATCTCAAAATTAATC
GAACCTCAAACCGGTTATAAACCGGTCTTAATTTCAAAGACTTGTAAACCGAAATAAAAA
TGTAAGAAAGAAATCCACCTGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 03-K114021-0022-120-B03C-8409
GenBank Accession LN487573 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K114021-0022-120-B03C-8409
CATTTCATTGAATATATCAGGTGGATTTCTTTCTTACATTTTTATTTCGGTTTACAGTCT
TTGAAATTAAGACCGGTTTATAACCGGTTTGAGGTTCGATTAATTTTGAGATCCTAAACA
TTTTTCATTTAATAGTAACAAATCCAACAAAAAAGATTGTAATTTAGAAAACCTAACACT
TGTCTTTTCTAAGCTTTCCATTTCTCAAGATCTCTACAAAAAAAATTCCATTTCTCTTTT
CGAGAGCCTTCTTCTTATAAAATATAAAAACAAGACTTAAGAATCGGCCATGTCGTCTTG
AATTTCGCTGGGAGCTATAAGTCCAGGAATCGAAAGCATTCTTTGTCTCTCTCTCTCCAT
TTGGGCAGCAGCTTCCTCAGCATATAAATCTTTGTTATCCTGAACAAAAATAATCCATTC
ATTAGAGTTTTTGATGAAATGTGGTATAAAAGAAGATACCAATCTAATCTGTAGCAATTA
CTTATATACCTGAGCAGAAAATTCCTTGGACTGTATAAGGAAGTCGCGTATGTTATCCTT
AAATCTGCCAACATCGTTTCT

Last Updated on 06.12.2018 16:11