Sequences from confirmation amplicon


Confirmation sequences for line 120B03 and hit At5g41520 are shown.
Line ID 120B03
Locus At5g41520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16609750
Primers used for PCR 21ry - 8474, show primer details
   
Sequence Name 28-K116132-0022-120-B03C-21ry
GenBank Accession LN498992 [GenBank]
Sequencing primer gene specific primer, 21ry: 5'-TGGAGGACAGTGTAAAAAGATTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K116132-0022-120-B03C-21ry
TTTTCGAGAAAGAAACAAAGTTCCAAAAGACACATTCGTTCTCAAATCAAAGGTACTATG
TAACCTTAAATAAGACCAAAAAGAAACAAGAAAGTCCTTTTCAAGGAAGATCAGATCCAG
CAGCACCACCACCGAAACCACCAGCTCCACGACCAAACCCTTGCCTAGCTCCACCAGCTC
CACCCTGTACATAACCAAAACATTTAATTACACACCAACATAATTGCATTGCATTCTAGC
AATCATGAAACCACCATTACTCATGATAGAAAGACCTAGTGGCAGAGCATCGAATATTTA
GGGAAGCCAAGTGCACAACAGATTAAAGCAAAGCAATTTACACAAGGTGCTTATCAGACA
GAGTTCATAAAATGATTTAGGGAACCTAAGAACACAACATGTTGTTGAAAGATAGTGGCT
TCAGAAGTTTCCTCAAAATTAAGCATGATACTAATGCGTAACAAATAAAAGTGTTCACAA
GAGTTTGACAAGAAAGGAGAGATTTGAAATTAAATGGAATACACCATACTGATAGTGAAT
ATATCTACATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 03-K116131-0022-120-B03C-8409
GenBank Accession LN487632 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K116131-0022-120-B03C-8409
ACACTTTTTATTTGTTACGCATTAGTATCACGCTTAATTTTTGAGGAAACTTCTGAAGCC
ACTATCTTTCAACAACATGTTGTGTTCTTAGGTTCCCTAAATCATTTTATGAACTCTGTC
TGATAAGCACCTTGTGTAAATTGCTTTGCTTTAATCTGTTGTGCACTTGGCTTCCCTAAA
TATTCGATGCTCTGCCACTAGGTCTTTCTATCATGAGTAATGGTGGTTTCATGATTGCTA
GAATGCAATGCAATTATGTTGGTGTGTAATTAAATGTTTTGGTTATGTACAGGGTGGAGC
TGGTGGAGCTAGGCAAGGGTTTGGTCGTGGAGCTGGTGGTTTCGGTGGTGGTGCTGCTGG
ATCTGATCTTCCTTGAAAAGGACTTTCTTGTTTCTTTTTGGTCTTATTTAAGGTTACATA
GTACCTTTGATTTGAGAACGAATGTGTCTTTTGGAACTTTGTTTCTTTCTCTAAAACCAT
CTCACAATATGATTGTTTTTTACGTTAGAATTTTATGAAGAAATGTTGGATCGTTACATG
AATCT

Last Updated on 06.12.2018 16:11