Sequences from confirmation amplicon


Confirmation sequences for line 121E11 and hit At1g79940 are shown.
Line ID 121E11
Locus At1g79940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30072356
Primers used for PCR 03jr - 8474, show primer details
   
Sequence Name 17-K113214-0022-121-E11C-8409
GenBank Accession LN493927 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K113214-0022-121-E11C-8409
TTGAGTAATTGGTTTATTATGTCTTTAGACTAGACCTTTAAGTAAACCAACCTAAAGAAC
CACTACTACTTATTTGGAGAACAGAATCCAACAAGATTCTCAGGAATCAAAAAGTGAGAG
AAAAATGCACATGATATGGAACTAGATATTAGTACTGCACTTGACACATTTGATAGTAGT
ATACCTTCCGTGCTATCTTTTTGACAACGGCATCACTGAAATGAGGGAGCTGTAAGAAAG
GGGAAATGCCTTCTGATGATACTCCAGAAGACTTCCTTGCACTAAGAGGAACAGCCTGCG
ATTTTGGATTTTTAGTTTATGTTCCTAGACAACAACACTAAGCACTTCTTAAACTTTCAG
GAAGAAGTAAATCTTGTGGAGAAAAGAGACATAAAGAACTGAAAGCTCACAAACAAATAA
AAACTAGAAGATAGGCGATGAGAGCAATTTAAGACCTGAACAATGCACTGGNATAGCT
   
Sequence Name 17-K113215-0022-121-E11C-03jr
GenBank Accession LN493928 [GenBank]
Sequencing primer gene specific primer, 03jr: 5'-GAGCTATCTCAGTGCATTGTTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K113215-0022-121-E11C-03jr
TTTTATTTGTTTGTGAGCTTTCGTTCTTTATGTCTCTTTTCTCCACAAGATTTACTTCTT
CCTGAAAGTTTAAGAAGTGCTTAGTGTTGTTGTCTAGGAACATAAACTAAAAATCCAAAA
TCGCAGGCTGTTCCTCTTAGTGCAAGGAAGTCTTCTGGAGTATCATCAGAAGGCATTTCC
CCTTTCTTACAGCTCCCTCATTTCAGTGATGCCGTTGTCAAAAAGATAGCACGGAAGGTA
TACTACTATCAAATGTGTCAAGTGCAGTACTAATATCTAGTTCCATATCATGTGCATTTT
TCTCTCACTTTTTGATTCCTGAGAATCTTGTTGGATTCTGTTCTCCAAATAAGTAGTAGT
GGTTCTTTAGGTTGGTTTACTTAAAGGTCTAGTCTAAAGACATATATAAACCAATTACTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCACGATTATTA

Last Updated on 06.12.2018 16:11