Genotyping and primer details


Genotyping and primer details for 121E11 - At1g79940
Line ID 121E11
Locus At1g79940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:30072356 of line 121E11 Chr1:30072356
   
Insertion allele amplicon 8474 - 03jr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03jr: 5'-GAGCTATCTCAGTGCATTGTTCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:30071887
   
wt amplicon (over InsPos) 16kj - 03jr
wt amplicon start Chr1:30071887
wt amplicon end Chr1:30072932
wt amplicon size 1046 bp
Locus specific primer 03jr: 5'-GAGCTATCTCAGTGCATTGTTCAG-3'
2nd locus specific primer 16kj: 5'-AGCAAGTCAAGTTGTATGTTCCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11