Sequences from confirmation amplicon


Confirmation sequences for line 122E08 and hit At3g49370 are shown.
Line ID 122E08
Locus At3g49370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18307432
Primers used for PCR 06qf - 8474, show primer details
   
Sequence Name 26-K115635-0022-122-E08C-06qf
GenBank Accession LN498065 [GenBank]
Sequencing primer gene specific primer, 06qf: 5'-AATGGTCCAAATCTAAACACAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K115635-0022-122-E08C-06qf
ACTTACTCCATAACAACAAAGACATTATCCGAATTCTCAAACACATCATAGAACTTAACC
ATGTGACTATGCCCAGACAAAGCTTTCAACAACTTCACCTCTCGACGAACATCTTCAATC
GACAAAGCTGATGTCATCTGAAAACACATTGTATCAACACAAAGAACAAATCTTTCGAAT
GAAACAGTAACCATTTCTATGTTCCAATAATCTTGGAACCAATCACATTGTAACAAACCT
TAGATTTAGAGATGATTTTGACTGCAACTGTTTGACCTTTGATCTTCCCTTTCTTAGCTT
TAGCCCAACAAGTATGACCAAAATGCCCTCGACCTACTTCTCTCCCAAGCTCATACTTAC
CTTCAAAAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 12-K115634-0022-122-E08C-8409
GenBank Accession LN491719 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K115634-0022-122-E08C-8409
GCTAAAGCTAAGAAAGGGAAGATCAAAGGTCAAACAGTTGCAGTCAAAATCATCTCTAAA
TCTAAGGTTTGTTACAATGTGATTGGTTCCAAGATTATTGGAACATAGAAATGGTTACTG
TTTCATTCGAAAGATTTGTTCTTTGTGTTGATACAATGTGTTTTCAGATGACATCAGCTT
TGTCGATTGAAGATGTTCGTCGAGAGGTGAAGTTGTTGAAAGCTTTGTCTGGGCATAGTC
ACATGGTTAAGTTCTATGATGTGTTTGAGGATTCGGATAATGTCTTTGTTGTTATGGAGT
AAGTATGTCTCTTTCATGCTTCTTATATATCAAAAGTTGTTTTATTTATAATCTCCTTGT
GTTT

Last Updated on 06.12.2018 16:11