Sequences from confirmation amplicon


Confirmation sequences for line 122E09 and hit At5g27610 are shown.
Line ID 122E09
Locus At5g27610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9769675
Primers used for PCR TM22 - 8474, show primer details
   
Sequence Name 47-K103693-0022-122-E09C-TM22
GenBank Accession LN506179 [GenBank]
Sequencing primer gene specific primer, TM22: 5'-TTGGATCATCATTCCTTAAACACA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K103693-0022-122-E09C-TM22
GTTTCTCTCCAGGAAGGATCAGGAAAAAACAAAACACCAATGCCTTCAGAGCTTATCACC
TCTTGTGTTGCATCTTGGCTCATGATGCAGGTAAAGGAAATAATTTTGTCTTGGACTAAC
GAAAGTGTAATCTAAATCAAGAATACCAAAGTTTTCACTTGATGCGGTTTTGATTAGAAC
TCTTTGTGTGTTATTTACCTGCAGATGATCTCAAAGAAGCAGTACCCACCAGCAGATGTG
GCTCAGCTAATGGATACAGTAGTGAATGACTTGCAGCCACGGTGTCCACAGAATATGCCG
ATATACAGAGAAATTCAAACTTGTATGGGATTGATCAAGACATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 63-K103694-0022-122-E09C-8409
GenBank Accession LN510972 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K103694-0022-122-E09C-8409
GCCATTTACAATTGAATGTCTTGATCAATCCCATACAAGTTTGAATTTCTCTGTATATCG
GCATATTCTGTGGACACCGTGGCTGCAAGTCATTCACTACTGTATCCATTAGCTGAGCCA
CATCTGCTGGTGGGTACTGCTTCTTTGAGATCATCTGCAGGTAAATAACACACAAAGAGT
TCTAATCAAAACCGCATCAAGTGAAAACTTTGGTATTCTTGATTTAGATTACACTTTCGT
TAGTCCAAGACAAAATTATTTCCTTTACCTGCATCATGAGCCAAGATGCAACACAAGAGG
TGATAAGCTCTGAAGGCATTGGTGTTTTGTTTTTTCCTGATCCTTCCTGTGAGATGAAAC
CGTTACTCATCGGCTCTGCTGTGTTTAAGGAAGATGGATCC

Last Updated on 06.12.2018 16:11