Sequences from confirmation amplicon


Confirmation sequences for line 122G02 and hit At4g26280 are shown.
Line ID 122G02
Locus At4g26280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13305298
Primers used for PCR DA73 - 8474, show primer details
   
Sequence Name 17-K117188-0022-122-G02C-DA73
GenBank Accession LN494033 [GenBank]
Sequencing primer gene specific primer, DA73: 5'-GAAGCAAACACAGCATTCAAAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K117188-0022-122-G02C-DA73
TGGAGAGTGGATGAACTTGAGAGACGAAGACTTAACGGAAGAAACAAAGACTCTAATCTC
TTCACTTTCTTCAGAGAAAGGGTACCTCGGTCGAAACCTCTGCAAGTACCAAGGCTCTTG
GTATTACTACAACTTTCTCCAAGGTGTTCTCAATTTCCAGAGAGGTTTTAAGCCTCAAGA
CACTGATATTATCGTCGCATCGTACCCAAAATCCGGCACCCTGTGGCTCAAGGCACTCAC
TGTCGCGCTTTTTGAGAGAACAAAGAACCCTTCTCATGATGATCCTATGAGCCATCCTCT
TCTATCCAACAATCCTCACAACCTCGTACCTGTCCTTGAGATGAATCTTTACCGGGATAC
CCAAACACCGGACTTGACCTAGCTCTCATCATCATCTCCAAGGTTGTTCTCTACTCACAC
GCCTTTCCACACGCTGCAAGTAGCTGTCAAGGACTCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCC
   
Sequence Name 02-K117187-0022-122-G02C-8409
GenBank Accession LN487209 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K117187-0022-122-G02C-8409
GGCGTGTGAGTAGAGAACAACCTTGGAGATGATGATGAGAGCTAGGTCAAGTCCGGTGTT
TGGGTATCCCGGTAAAGATTCATCTCAAGGACAGGTACGAGGTTGTGAGGATTGTTGGAT
AGAAGAGGATGGCTCATAGGATCATCATGAGAAGGGTTCTTTGTTCTCTCAAAAAGCGCG
ACAGTGAGTGCCTTGAGCCACAGGGTGCCGGATTTTGGGTACGATGCGACGATAATATCA
GTGTCTTGAGGCTTAAAACCTCTCTGGAAATTGAGAACACCTTGGAGAAAGTTGTAGTAA
TACCAAGAGCCTTGGTACTTGCAGAGGTTTCGACCGAGGTACCCTTTCTCTGAAGAAAGT
GAAGAGATTAGAGTCTTTGTTTCTTCCGTTAAGTCTTCGTCTCTCAAGTTCATCCACTTC
TCCATTTTTCTTTTGTTTTTACTTTTAGCGACTTATTTTGAATGCTGTGTTTGCTTC

Last Updated on 06.12.2018 16:11