Sequences from confirmation amplicon


Confirmation sequences for line 122H12 and hit At3g17920 are shown.
Line ID 122H12
Locus At3g17920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6141539
Primers used for PCR 06uj - 8474, show primer details
   
Sequence Name 56-K117671-0022-122-H12C-06uj
GenBank Accession LN509221 [GenBank]
Sequencing primer gene specific primer, 06uj: 5'-ATCGAAGAATATTTTGGACACACC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K117671-0022-122-H12C-06uj
GCTTATCAGATGAATCAAACTCACGAACAAATTGAATCAAATGAATTTGAAGTTATGAAC
AAATGTCAAGTTCTAAATATTTACGGCTCATCTCTCACCCAAATGCCTTACGAACAATGG
GAATTCTGTCGAATCTGGGTGAAGTCCATTTACTAGAGCCACAAATTTGGGTACACACTC
AGGGCTGAGTAACTTGAGCTTCCATGTTACTGAATTCACTGTCCTCTTTTGGCTGCTTTG
AAATGTGGAATCCTTCTGCTTGACCTTCAGTGACAAGCACGAGCTTCCTCTTGATTCAAC
AACCTGGATAACACCCCAATTTGCACATAAAAAACTGACATAAGCGAGAGAAAGGGAAAG
AATAAACAAACTTGTTACCATCTCAAATATGTCGGAGATGGAGCAGCTTGAGTCAAGTGA
GAAATATGGGGATGAAGAAGCATCATCTGTTGGCAGAAAACTCAGTTGCCTGAAGTCTTC
ATTGCATATGAAAAGATGTCCACCCGCCACAAAGAGTGACCGTGGAAGCCATGACACTTC
TGTTCAGTTTAACACAAAAAAATTGATTAACACAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 08-K117670-0022-122-H12C-8409
GenBank Accession LN489913 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K117670-0022-122-H12C-8409
ATTTCATTGTGTTATCAATTTTTTTGTGTAAACTGAACAGAAGTGTCATGGCTTCCACGG
TCACTCTTTGTGGCGGGTGGACATCTTTTCATATGCAATGAAGACTTCAGGCAACTGAGT
TTTCTGCCAACAGATGATGCTTCTTCATCCCCATATTTCTCACTTGACTCAAGCTGCTCC
ATCTCCGACATATTTGAGATGGTAACAAGTTTGTTTATTCTTTCCCTTTCTCTCGCTTAT
GTCAGTTTTTTATGTGCAAATTGGGGTGTTATCCAGGTTGTTGAATCAAGAGGAAGCTCG
TGCTTGTCACTGAAGGTCAAGCAGAAGGATTCCACATTTCAAAGCAGCCAAAAGAGGACA
GTGAATTCAGTAACATGGAAGCTCAAGTTACTCAGCCCTGAGTGTGTACCCAAATTTGTG
GCTCTAGTAAATGGACTTCACCCAGATTCGACAGAATTCCCATTGTTCGTAAGGCATTTT
GGTGAGAGATGAGCCCGTAATATTTAGAACTTGACATTTGTTCATAACTTCA

Last Updated on 06.12.2018 16:11