Sequences from confirmation amplicon


Confirmation sequences for line 123E08 and hit At1g23140 are shown.
Line ID 123E08
Locus At1g23140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8202617
Primers used for PCR TH08 - 8474, show primer details
   
Sequence Name 39-K103676-0022-123-E08C-TH08
GenBank Accession LN503326 [GenBank]
Sequencing primer gene specific primer, TH08: 5'-TGCTTCATTCTTCTTCTGCAACTA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K103676-0022-123-E08C-TH08
GACAAAGACAAGAGTCCGAGGAGACATGGAGAATCTGGTGGGACTTCTTAGAATCAGAGT
GAAAAGAGGGATTAATCTTGTTAGTCGTGATTCTAATACCAGCGACCCTTTCGTCGTCGT
AACCATGGGTTCTCAGGTATCTCTCTTATGTTCTCTGTTCTCTGTGTTATAAAGATCGTG
TTTTGATCATCTGAGGTAAGATAGATTAGCCCATTATACTCCGTAGATTCCAATTTTGAA
AAAGAGGAAACTTTAGAAGATTTGGAAATTACTCTGTTTTTGATGGAACTTATAATGGAC
TTTTAAGAGACTTGTGATGATATCAATCGATTTGTCTCAGAAATTGAAGACACGTGGTGT
GGAAAATAGCTGCAACCCTGAGTGGGACGATGAATTGACGCTTGGAATCAATGATCCTAA
TCAACATGTGACTCTCGTGAGTTAGTTCTTGATCTTCTTGAAATGATTCTTGATCTTAAA
GGAGTCTTTTGGTTTTGGTGAAGAGATTATGTTTCTGAGTTTTTGTTTGTTATGTTTCCT
TGTAGGAAGTGTATGATAAAGATACGTTCACTTCCCATGACTTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
   
Sequence Name 55-K103677-0022-123-E08C-8409
GenBank Accession LN508785 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K103677-0022-123-E08C-8409
GCCATTTACAATTGAAGTCATGGGAAGTGAACGTATCTTTATCATACACTTCCTACAAGG
AAACATAACAAACAAAAACTCAGAAACATAATCTCTTCACCAAAACCAAAAGACTCCTTT
AAGATCAAGAATCATTTCAAGAAGATCAAGAACTAACTCACGAGAGTCACATGTTGATTA
GGATCATTGATTCCAAGCGTCAATTCATCGTCCCACTCAGGGTTGCAGCTATTTTCCACA
CCACGTGTCTTCAATTTCTGAGACAAATCGATTGATATCATCACAAGTCTCTTAAAAGTC
CATTATAAGTTCCATCAAAAACAGAGTAATTTCCAAATCTTCTAAAGTTTCCTCTTTTTC
AAAATTGGAATCTACGGAGTATAATGGGCTAATCTATCTTACCTCAGATGATCAAAACAC
GATCTTTATAACACAGAGAACAGAGAACATAAGAGAGATACCTGAGAACCCATGGTTACG
ACGACGAANGGGTCGCTGGTATTAGAATCACGACTAACAAGATTAATCCCTCTTTTCACT
CTGATTCTAAGAAGTCCCACCAGATTCTCCATGTCTCCTCGGACTCTTGTCTTTTGTCTT
GTACTTGGATTCTTAGAAGAGAAACTAGTTGCAGAAG

Last Updated on 06.12.2018 16:11