Sequences from confirmation amplicon


Confirmation sequences for line 124D02 and hit At5g17730 are shown.
Line ID 124D02
Locus At5g17730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5852952
Primers used for PCR BK83 - 3144, show primer details
   
Sequence Name 01-K104986-0022-124-D02C-3144
GenBank Accession LN486568 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K104986-0022-124-D02C-3144
TTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAGCCCATTGTCAGGGCTCTTAACTTGCA
TAGATGGGTTATGGTCGAGCTGTGGAGATGAACGCATTGTAATCTTCACCACAACCCACA
AGGAGAGGCTCGATCCGGCATTGCTTAGGCCAGGACGTATGGATATGCATATTCACATGG
GACATTGTTGTTTTGATGTATTCAAGACTTTGGCATCTAACTACTTGGGCTTGTCTCACG
ATGACCCTCACCATCTTTACCCGGAAATCGAGCGTTTGATAAAAGGAGAAGTGTTGACTC
CAGCTCAAGTTGCAGAGGAGCTAATGAAAAATGAGGATCCTGATGTGGCGCTCGAGGGTT
TGGTTAAGGTTCTCAAAAGGAAGAGGTTAGAGTTGGAGAAGTATGATGGAGAAACTGGAA
GAGGAGGATTGAGGAAACCGGAATTGCAATTCTTTTTATGAGTTAACATTGTGGCATCCT
   
Sequence Name 17-K104985-0022-124-D02C-BK83
GenBank Accession LN493844 [GenBank]
Sequencing primer gene specific primer, BK83: 5'-ATAAATAAACAAACAGGACGCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K104985-0022-124-D02C-BK83
TTCGGTTTCTCAATCCTCCTCTTCAGTTTCTCCATCATACTTCTCCAACTCTAACCTCTT
CCTTTTGAGAACCTTAACCAAACCCTCGAGCGCCACATCAGGATCCTCATTTTTCATTAG
CTCCTCTGCAACTTGAGCTGGAGTCAACACTTCTCCTTTTATCAAACGCTCGATTTCCGG
GTAAAGATGGTGAGGGTCATCGTGAGACAAGCCCAAGTAGTTAGATGCCAAAGTCTTGAA
TACATCAAAACAACAATGTCCCATGTGAATATGCATATCCATACGTCCTGGCCTAAGCAA
TGCCGGATCGAGCCTCTCCTTGTGGGTTGTGGTGAAGATTACAATGCGTTCATCTCCACA
GCTCGACCATAACCCATCTATGCAAGTTAAGAGCCCTGACAATGGGCTTAAACCGAAGGC
GGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTA
TATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGA
GATATC
Primers used for PCR BK84 - 8474, show primer details
   
Sequence Name 02-K104986-0022-124-D02C-8409
GenBank Accession LN487025 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K104986-0022-124-D02C-8409
ATCCAGTATTGGTTGCAAGCATTTCATGATGATTTATAAATTAATACTAGTTTTTAATGA
AAATAAAGACCAAACCTTAGTATCATCTTGAGTCTTAGGTTGCAACCTTGTATGCAAATC
CACGGCGCAATCTATATCTTCTACTAAAAGAATGGAGCTGTTGGTAGTACCGAGAAGTAA
TCTCCTTAGATCAGCGTCCTCCCTCACGCTTGCAAGCTGGAGATCATAGATATCAAACTT
GAGGTAGTTAGCAATGGCTGCAACTAGGCTAGTCTTCCCAGTCCCAGGTGGTCCATACAG
CAAGTAACCCCTTTTCCAAGGTTTCCCAACTCTTTTGTAAAAATCTTTCCTTCTGATGAA
CCGGTCAAGATCTCCCATCACAGACCGCTTTAGCTCTTCGTTCATAGCCATGGTGTCAAA
TGTTGACGGGTGCTCGAGGTTCACGGATTGCCATTTTAGATACATACAACAGTAGGAGTA
CATCTTGAGGATTTTCCTCTCGTTGTTGATCACCTTTGCCTTGCTCTCCACGTAAGGTAT
ATAAGAATTCAAGACCAACTCTGTG
   
Sequence Name 18-K104985-0022-124-D02C-BK84
GenBank Accession LN494297 [GenBank]
Sequencing primer gene specific primer, BK84: 5'-TTGAAAAGAAACACACAGAGTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K104985-0022-124-D02C-BK84
AGGCAAGGTGATCAACAACGAGAGGAAAATCCTCAAGATGTACTCCTACTGTTGTATGTA
TCTAAAATGGCAATCCGTGAACCTCGAGCACCCGTCAACATTTGACACCATGGCTATGAA
CGAAGAGCTAAAGCGGTCTGTGATGGGAGATCTTGACCGGTTCATCAGAAGGAAAGATTT
TTACAAAAGAGTTGGGAAACCTTGGAAAAGGGGTTACTTGCTGTATGGACCACCTGGGAC
TGGGAAGACTAGCCTAGTTGCAGCCATTGCTAACTACCTCAAGTTTGATATCTATGATCT
CCAGCTTGCAAGCGTGAGGGAGGACGCTGATCTAAGGAGATTACTTCTCGGTACTACCAA
CAGCTCCATTCTTTTAGTAGAAGATATAGATTGCGCCGTGGATTTGCATACAAGGTTGCA
ACCTAAGACTCAAGATGATACTAAGGTTTGGTCTTTATTTTCATTAAAAACTAGTATTAA
TTTATAAATCATCATGAAATGCTTGCAACCAATACTTGGATCCTATGTGTAGATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATT

Last Updated on 06.12.2018 16:11