Sequences from confirmation amplicon


Confirmation sequences for line 124E07 and hit At1g17790 are shown.
Line ID 124E07
Locus At1g17790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6125947
Primers used for PCR 20ra - 8474, show primer details
   
Sequence Name 79-K115895-0022-124-E07C-20ra
GenBank Accession LN513868 [GenBank]
Sequencing primer gene specific primer, 20ra: 5'-AATCTATGACAATCCCTGTGGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K115895-0022-124-E07C-20ra
GCTGAGAGAGGAAGCCCTGTTAACACAGGGACCTCACCTTGGAAGAGAAACGGAGACTCA
GTGAAGAGCTTCAAGACTTGCCTTATGACAAACTAGAGACTGTTGTTCAGATCATAAAGA
AGAGCAATCCAGAGCTCTCTCAAAAAGATGATGAGATTGAGCTGGATATTGATAGTCTTG
ATATCAACACGCTTTGGGAGCTTTATAGATTTGTGACTGGGTATAAGGAGAGCTTGAGCA
AGAAAAACGAGGCTCATGGATTTGGTTCAGAAAGAGATGCTGAATCTGTTCACAATAGTA
TTCAAGAACCTGTAAGATTTCCATAGCTAATTGTTTATTCAGGTCTTCTGCTCTTACTGT
ATTTGGCTAATATCATGGTGTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 49-K115894-0022-124-E07C-8409
GenBank Accession LN506948 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K115894-0022-124-E07C-8409
CATTTCATTGAATATATCCTGAACCCATGATATTAGCCAAATACAGTAAGAGCAGAAGAC
CTGAATAAACAATTAGCTATGGAAATCTTACAGGTTCTTGAATACTATTGTGAACAGATT
CAGCATCTCTTTCTGAACCAAATCCATGAGCCTCGTTTTTCTTGCTCAAGCTCTCCTTAT
ACCCAGTCACAAATCTATAAAGCTCCCAAAGCGTGTTGATATCAAGACTATCAATATCCA
GCTCAATCTCATCATCTTTTTGAGAGAGCTCTGGATTGCTCTTCTTTATGATCTGAACAA
CAGTCTCTAGTTTGTCATAAGGCAAGTCTTGAAGCTCTTCACTGAGTCTCCGTTTCTCTT
CCAAGGTGAGGTCCCTGTTGTTAACAGGTGCTTCCTCTTCTTCAGCTTTCTCTGGAGCAG
TAATTACAGCTTCAGGTTCCACAGGGATTGTCATAGAT

Last Updated on 06.12.2018 16:11