Sequences from confirmation amplicon


Confirmation sequences for line 124E07 and hit At1g18830 are shown.
Line ID 124E07
Locus At1g18830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6490276
Primers used for PCR 12sm - 8474, show primer details
   
Sequence Name 26-K116405-0022-124-E07C-12sm
GenBank Accession LN498083 [GenBank]
Sequencing primer gene specific primer, 12sm: 5'-CCAACAAATAGTTCGGTTGTCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K116405-0022-124-E07C-12sm
ACTCCATTGCAATCCACCTGTTGTATAACAAGTATCACCACTCTGTTTCAACCAATGCAA
GGGTACATTCTATATAGTAAAAGAGATGGAGATACCTCTTTGGTGACCAACAAATGTGCG
AACAGGTGATTGTAGATACCTTATGTCCAAAAGCTGACAGAAAACATCTTTAGAGACCAA
TGAAAAGAGCCAAATTTGATTGAACTTGAAAAAATAAAAATTAATATGATCTGATTACCT
TCACATTCGGTGAACTATCTTCATCTGAAGCAACCAAAATCTGATTAAAATGATCAGGAT
CCCATTGCAAAACTGAGCACCTAACGGTAGTTTTCAAGCTGTTGATTGATAATGGCTAGA
TCAGGTTTTGATAAGAGATCACAGAGGATTTCTAATATAACAAACTAAAAATAAATCAGA
ACCAATATTCAATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAACGTTATTAT
   
Sequence Name 16-K116404-0022-124-E07C-8409
GenBank Accession LN493583 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K116404-0022-124-E07C-8409
GCATTTCATTGAATTGATATTGGTTCTGATTTATTTTTAGTTTGTTATATTAGAAATCCT
CTGTGATCTCTTATCAAAACCTGATCTAGCCATTATCAATCAACAGCTTGAAAACTACCG
TTAGGTGCTCAGTTTTGCAATGGGATCCTGATCATTTTAATCAGATTTTGGTTGCTTCAG
ATGAAGATAGTTCACCGAATGTGAAGGTAATCAGATCATATTAATTTTTATTTTTTCAAG
TTCAATCAAATTTGGCTCTTTTCATTGGTCTCTAAAGATGTTTTCTGTCAGCTTTTGGAC
ATAAGGTATCTACAATCACCTGTTCGCACATTTGTTGGTCACCAAAGAGGTATCTCCATC
TCTTTTACTATATAGAATGTACCCTTGCATTGGTTGAAACAGAGTGGTGATACTTGTTAT
ACAACAGGTGTGATTGCAATGGAGTGGTGTCCAAGTGACAGCTTGTATCTACTTACCTGT
GGGAAAGACAACCGAACTATTTGTTGG

Last Updated on 06.12.2018 16:11