Sequences from confirmation amplicon


Confirmation sequences for line 125A01 and hit At1g31760 are shown.
Line ID 125A01
Locus At1g31760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11373399
Primers used for PCR 05fp - 3144, show primer details
   
Sequence Name 50-K111394-0022-125-A01C-05fp
GenBank Accession LN507209 [GenBank]
Sequencing primer gene specific primer, 05fp: 5'-ACAATTCCTTCTCTAAAGCAACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K111394-0022-125-A01C-05fp
AAGTAAACCTTAGAAGCTCCAAATCAAACAAACCTGGAGATTGTGAAGCTTCACATACTC
CCAAACTTTCTTCAGAGCGGAACCACGAGTGAGTTCCCTTTCTCCGGCGAAGCTAGCTAG
CTGCTGTGACACCGGACGAGTCTTGAATATCCCTTTTCCTTCTCCGGCTACAGTAACGGC
CTCAACAGTCGCCGCCTTCGACATCAGTGTCCTGAACCCTCCCAAAACCCTCTCAAACAC
TGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCC
AAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCG
TCATCCCTTACGTCAGTGGAGATATC
   
Sequence Name 34-K111393-0022-125-A01C-3144
GenBank Accession LN501462 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K111393-0022-125-A01C-3144
CAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGAT
CAGATTGTCGTTTCCCGCCTTCGGTTTAACTATCAGTGTTTGAGAGGGTTTTGGGAGGGT
TCAGGACACTGATGTCGAAGGCGGCGACTGTTGAGGCCGTTACTGTAGCCGGAGAAGGAA
AAGGGATATTCAAGACTCGTCCGGTGTCACAGCAGCTAGCTAGCTTCGCCGGAGAAAGGG
AACTCACTCGTGGTTCCGCTCTGAAGAAAGTTTGGGAGTATGTGAAGCTTCACAATCTCC
AGGTTTGTTTGATTTTGGAGCTTTCTAAGGTTTTTACTTTGCGTAGATCTGTTTATGAAC
TTAGGGATTTGGTTGCTTTAGAGAGGGAATTG

Last Updated on 06.12.2018 16:11