Sequences from confirmation amplicon


Confirmation sequences for line 125B11 and hit At2g47010 are shown.
Line ID 125B11
Locus At2g47010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19318528
Primers used for PCR 06vh - 8474, show primer details
   
Sequence Name 46-K117914-0022-125-B11C-06vh
GenBank Accession LN505996 [GenBank]
Sequencing primer gene specific primer, 06vh: 5'-CTCAAAAATGGAAACTACGACACA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K117914-0022-125-B11C-06vh
CAAAGATCCCCCTTTCATCAACCCGGATTATTCCCCTGTTTCGGCAGCGGCCCTTTGAAC
CAACCGACGCTGAACCACGGAAAAACCGAGCTTCATAGAGATGGTCAAACCATGAAGGGA
TGGTTCAATGGTACGTACGAGCAAGGGGCAGATTTTGGAAATGGGTTGGATGGGATAAGT
TATTACGAGGTTGTGTGGGAGAAGAGAGTAGGCGTTGGTGGTTGGGTATTTAAACATAAG
CTTAAGACTTCAGCTAAGTACCCATGGCTTATGCTTTACCTTAGAGCTGATGCCACTAAA
GGATTCTCTGGTGGATACCATTATGACACTAGAGGAATGCTCAAAACAGTAAGTAATTAA
CACTAACTTTAAACCTTTTTGAAAAGTTTTTTTCTTTCTTGTCAAAAAGAAATTGATTGA
TCTTTTGTGATGAAATATTGAAATGTGTTGTGTTTTTGTATTATCTTCCAAAAATGGCTT
CTTGTCCGGGAAATCTACTTGGATCAGCAATGAATATGATGGTTAATATGAAAAAAAAGA
ACTTTTTCCTACTTATTTTTTTACAATCCAAAAATGTA
   
Sequence Name 06-K117913-0022-125-B11C-8409
GenBank Accession LN488997 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K117913-0022-125-B11C-8409
ATTTTTGGAGCTAATACAAAAACACAACACATTTCAATATTTCATCACAAAAGATCAATC
AATTTCTTTTTGACAAGAATGAAAAAAACTTTTCAAAAAGGTTTAAAGTTAGTGTTAATT
ACTTACTGTTTTGAGCATTCCTCTAGTGTCATAATGGTATCCACCAGAGAATCCTTTATT
GGCATCAGCTCTAATGTAAAACATAAGCCATGGGTACTTAGCTGAAATCTTAACCTTATG
TTTAAATACCCAACCACCAACGCCGACTCTCTTCTCCCACACAACCTCATAATAACTTAT
CCCATCCTACCCATTTCCAAAATCTGCCCCTTGCTCGTACGTACCATTGAACCATCCCTT
CATGGTTTGACCATCTCTCTGAAGCTCGGTTTTTCCGTGGTTCAGCGTCGGTTGGTTCAT
ACAACCGCTGCCGAAACAGGGGAATAATCCGGGTTGATTGAAAGGTGGGATCTTTTTGCC
GTTTTTAGGGCAAAGAGCAGATTTTGTGTCGTATTTTCCATTTTTAAGAAAAAAAAGGAT
CTTTTTCCTTTTTTTTTTTTAAAAAATAA

Last Updated on 06.12.2018 16:11