Sequences from confirmation amplicon


Confirmation sequences for line 125C08 and hit At4g36820 are shown.
Line ID 125C08
Locus At4g36820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17347446
Primers used for PCR 04uz - 3144, show primer details
   
Sequence Name 47-K117497-0022-125-C08C-04uz
GenBank Accession LN506319 [GenBank]
Sequencing primer gene specific primer, 04uz: 5'-TGGGGGTCAATATATCTTTCTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K117497-0022-125-C08C-04uz
ATCCAGAATCTTGGGAACAGCACCTGAAGGCTTGGGATTAAACANGCTTTTTCAGCTCAT
CATACCTCCCAACATCAAAATCCTCAGATTGCATCAGTTTCCTTTGTTTAGCCTCAAACC
TGCTTTGGTAAAAACCTTGAAAGGAAGGCTCTCTCGATGTCTTGAGGAAAAATGTGTAAC
CCGCCATGAAATATACCGATGAGACATAGAAACAGATCGGTTCCATAACATCCCATGTCA
GGTCCCAAAACGTTAGCCTCATAAACCCTGCGGTTTGGATGATCAAGTAACCCAAACCAG
CCCAAAGCTCTCTTCTCACCAAAGAATGTGCTTTTTGGTCAATGACCGTCTTTATAGCTT
CAAGCTCATTTAGCTCCTTTCTTCTTGGGTCATTCGGGTTATGGATTTGTGATAAAGGAA
GAAGCCCCTCTATGGATTTGGTTAACTGATCAACACACAAGACCTTATCAGAGAATTGCA
TAGACAAAAAAGAATTCAAATGGTAAAAAAAAGCAAAGAGGAGGAAGAAGTTACCAATAT
GGCTTCCCATAACGATAACGTTTATCTCGTATGATGGTCAATATGGAGAAACTTATTTTC
GTAAACCTATAAGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACC
CTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGT
GGGATTGTGCGTCATCCCTTACGTCAGTGGAGATA
   
Sequence Name 15-K117496-0022-125-C08C-3144
GenBank Accession LN493155 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K117496-0022-125-C08C-3144
CTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATT
GTCGTTTCCCGCCTTCGGTTTAACTATCTTATAGGTTTACGAAAATAAGTTTCTCCATAT
TGACCATCATACGAGATAAACGTTATCGTTATGGGAAGCCATATTGGTAACTTCTTCCTC
CTCTTTGCTTTTTTTTACCATTTGAATTCTTTTTTGTCTATGCAATTCTCTGATAAGGTC
TTGTGTGTTGATCAGTTAACCAAATCCATAGAGGGGCTTCTTCCTTTATCACAAATCCAT
AACCCGAATGACCCAAGAAGAAAGGAGCTAAATGAGCTTGAAGCTATAAAGACGGTCATT
GACCAAAAAGCACATTCTTTGGTGAGAAGAGAGCTTTGGGCTGGTTTGGGTTACTTGATC
ATCCAAACCGCAGGGTTTATGAGGCTAACGTTTTGGGACCTGACATGGGATGTTATGGAA
CCGATCTGTTTCTATGTCTCATCGGTATATTTCATGGCGGGTTACACATTTTTCCTCAAG
ACATCGAGAGAGCCTTCCTTTCAAGGTTTTTACCAAAGCAGGTTTGAGGCTAAACAAAGG
AAACTGATGCAATCTGAGGATTTTGATGTTGGGAGGTATGATGAGCTGAAAAAGCTGTTT
AATCCCAAGCCTTCAGGTGCTGTTCCCAAGATTCTTGGATCTCTACAGAATTGATCATTG
GTGTCTCGTTGTCCTACTAACCTGAGAAAGAT

Last Updated on 06.12.2018 16:11