Sequences from confirmation amplicon


Confirmation sequences for line 125C09 and hit At1g09950 are shown.
Line ID 125C09
Locus At1g09950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3240653
Primers used for PCR 04be - 8474, show primer details
   
Sequence Name 02-K107367-0022-125-C09C-04be
GenBank Accession LN487058 [GenBank]
Sequencing primer gene specific primer, 04be: 5'-TAACCAGTTGACATTTGTTTGCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K107367-0022-125-C09C-04be
ATTGTTTAAATTAGNAGCAGCGATATGTTTTTTTTTTTAGAATATAAAACCATTTATGGT
TTCCAAAAAACCATTTAATTTTCCCTAAATGGGAAGGAATAAATGAATAAGGCCTCAACC
CCCAATGTAAATAAACTTGGGAAAAATTCAAATCTTTTACAACTTGTCAACATACAATAC
CTTTGTGTATGTAAACTAAACTTGGATTAAAAATAGTTCAACCCTTTCTTCATTTAAAGC
ATTGTTTGTTTATTTTCTTAGGCTTATCACGTATAAAAAACCCCATCAAAGCTAACTTTT
ACCCCCTCATCGGGAAATCTCTAGAAACCCTCTAAAGTCAACCCCTTGCCTAAAAATAAC
TTTGACCATTTTACCCAAAAAAAATATGCATCTTTTGAAATATGGAACCCCCGGTTTTCA
ATTTGCAAAACTACAAACTCTTTTTGTACCCCGTTGTTCACTGTTGTGGGCCCAAGGCTT
TAACCCCATTTTTGTGTTGATAAAGGGAAAAAAAGTAATATTTTTTCAACAATTACTTGT
CACCAAGTTAGTAAAGTCCTATAAATGACTGGGCGACCCCTTCAAAACCTACTTAGGATT
TTAAAACCAAGACCGGGGTACTTTTAACATTTTTGGGCCTGATTGTAAAAGTAACTGATG
AAATTTAGTAAAACCTGATTTAGGGAAAAACCGAACAACCAAAGGGAAATTTTCAATTGT
AAATGGCTTCTTGTCCGGGAAATCTACTTGGATCACCAATGAGTATGATGGTCAATTTGG
AGAAAAAGAAAGAGTATTTCCCATTTTTTTTTCAATTCAAAAATGTA
   
Sequence Name 18-K107368-0022-125-C09C-8409
GenBank Accession LN494331 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K107368-0022-125-C09C-8409
GAGCCATTTACAATTGAATATTTCCTTTTGTTTGTTCGTTTCTTCCTTAAATCAGTTCTT
ACTAAATCTCATCAGTTACTTTTACAATCAGACCAAAAAATGTCAAAAGTACACAGTACT
TGCTTTCAAAATCCTACGTAGCTTATGATGTGGTCGCCATGTCATTTATAGTACTTTACT
AACTTGCTGACATGTAATTGTTGAAAAAATATTACTTTTATTCCATTTATCAACACAAAA
CTGGGCTTAAAGCATTGGGCCAACAACAGTGAACAACGGGATACAAAAAGAGTTCGTAGT
TCTGCAAATCGAATACAGGAGGTCCAATATTTCAAAAGATGCATATTTTTCTTGGCTAAA
TTGGTCAATGTTATTGTTAGGCATGGCGTTGACTTTAGAGGCTTCCTAGAGATTGCCAGA
TGAGGGAGTAAAAGTCAGCTTTGATGGGCTTTTTCATACGTGATAAGCCTAAGAATATAA
ACAAACAATGCTCTAACTGAAGAATGGATTGAACTATTTTTAATCCAAGTCTAGTTTACA
TACACAAAGGTATTGTATGTTGACATGTTGTAATAGATTCGAATTTTCCACAAGTCTATC
TACATTGTGGTTTGAGTCATTATTCATTTATTCCTTCCAATTTAGTGAAACTTAAATGAT
TATCTGAAAACAATAAATGATTATATATACTAAAAAAAAAAAACATATCGCTGCTACTAT
TTTTAAACATTTCCGGAAGGAGGGAAGAAAAAGAACATTTTTGCAAACAAT

Last Updated on 06.12.2018 16:11