Sequences from confirmation amplicon


Confirmation sequences for line 125E09 and hit At4g15770 are shown.
Line ID 125E09
Locus At4g15770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8978317
Primers used for PCR 09qv - 8474, show primer details
   
Sequence Name 63-K115779-0022-125-E09C-09qv
GenBank Accession LN511057 [GenBank]
Sequencing primer gene specific primer, 09qv: 5'-AGGTTTGGTCCTAAATCCCTAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K115779-0022-125-E09C-09qv
AGAGCTTCTCTTCTTTCTCCATTATTCATCACTTTCTTCCCCGAAGATGCGTCCTTTAGA
CGAGACCGAGACAACCGTCGTCTTCGAGAAGCTCTTCAAATTCGTCGGCAACAACCTCAA
GAAGATCGTCGAGAACCCATCAGACGAAGGACCCGAATCTACACCCGGCAGCTACTGCTT
CCGCCTCCAGAAAAACAGAGTCTACTACGTCAGCGAAGCCCTCGTGAAGCGAGCCACAAA
CATCTCCAGGAAAAACCTAGTCTCCTTCGGAACCTGCATCGGGAAATACACTCACGCCGG
TAGTCAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCACGNTTATTATA
   
Sequence Name 15-K115778-0022-125-E09C-8409
GenBank Accession LN493115 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K115778-0022-125-E09C-8409
TTACATTGAATTATTGACTACCGGCGTGAGTGTATTTCCCGATGCAGGTTCCGAAGGAGA
CTAGGTTTTTCCTGGAGATGTTTGTGGCTCGCTTCACGAGGGCTTCGCTGACGTAGTAGA
CTCTGTTTTTCTGGAGGCGGAAGCAGTAGCTGCCGGGTGTAGATTCGGGTCCTTCGTCTG
ATGGGTTCTCGACGATCTTCTTGAGGTTGTTGCCGACGAATTTGAAGAGCTTCTCGAAGA
CGACGGTTGTCTCGGTCTCGTCTAAAGGACGCATCTTCGGGGAAGAAAGTGATGAATAAT
GGAGAAAGAAGAGAAGCTCTCTGTTTCTAAGAGGGATTGTGTTTTTTTAGGGTTTTAGGG
ATTTAGGACCAAACCTAAAAAAACACNATCCCTCTTAGAAACAGAGAGCTTCTCTTCTTT
CTCCATTATTCATCACTTTCTTCCCCGAAGATGCGTCCTTTAGACGAGACCGAGACAACC
GTCGTCTTCGAGAAGCTCTTCAGATTCGTCGGCAACAACCTCAAGAAGATCGTCGAGAAC
CCATCAGACGATGGACCCGAATCTACACCCGGCAGCTACTGCTTCCGCCTCCAGAAAAAC
AGAGTCTACTACGTCAGCGAAGCCCTCGTGAAGCGAGCCACAAACATCTCCAGGAAAAAC
GNAGTCTCCTTCGGAACCTGCATCGGGAAATACACTCACGCCGGTTGTCAGTATATTCAT
TTGTACATGGCTTCATGTC

Last Updated on 06.12.2018 16:11