Sequences from confirmation amplicon


Confirmation sequences for line 125E11 and hit At3g05050 are shown.
Line ID 125E11
Locus At3g05050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1409127
Primers used for PCR HN19 - 8474, show primer details
   
Sequence Name 70-K035348-022-125-E11C-HN19
GenBank Accession LN512326 [GenBank]
Sequencing primer gene specific primer, HN19: 5'-AAAGCTACGATTTTTATTGGCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K035348-022-125-E11C-HN19
ATTTTCATTTCAAAAATTTATATTACGTCAACTCTTACAATAATGACGTAGGCTTCTAGA
ACAATAAATATAAGGATCAAATAAATAATTTACATAGAATGTAAATTAATTCCTCTCATT
TCTGTGTTTTGTCAATTCTCTTGTACAATACACATAACAACCAAATAAAGAACATATAAC
CCACCAAAAAGATAAAATTTGGAATTTTGTTTTTTTCTCAATCTATCAAAAAGGAAACTT
TTGAAATTTGGAGATGAATAATAATTATTATCTCAAACAACGATTCTTTTGCCTTTGACC
TTAGAAGCCATTCTTCTCTCTTTGTTAATATCGTTTCTTCTCCTTGTGGTTGAGTCAGGT
GCTCCAATGGGAGCCAAAGGGCCTGACCAAGTTTGAAATGGCTCTTTGGAGTATGATGAT
GTGAATGAGTTTGTGCTATACGGTATCTCTGATGGATCCAGTCGCCTTGAAGATCCCACC
TGAAAACCGAGTGACCCGTCTTGATGCGGTGGAGGAAACTTCTCGCTCTTGCTCTTTGCA
TTCGCGTGTGTGATCATACGCATCCTCTGTTTTAAGAGAACAAGTTCAAAGTACTCAAGA
TATTACCAATGAGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACG
ACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGA
GATCTTTATNTCGACGTGTCTA
   
Sequence Name 22-K035348-022-125-E11C-8409
GenBank Accession LN496022 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K035348-022-125-E11C-8409
GAGCCATTTAAATTGAATAACTCATTGGTAATATCTTGAGTACTTTGAACTTGTTCTCTT
AAAACAGAGGATGCGTATGATCACACACGCGAATGCAAAGAGCAAGAGCGAGAAGTTTCC
TCCACCGCATCAAGACGGGTCACTCGGTTTTCAGGTGGGATCTTCAAGGCGACTGGATCC
ATCAGAGATACCGTATAGCACAAACTCATTCACATCATCATACTCCAAAGAGCCATTTCA
AACTTGGTCAGGCCCTTTGGCTCCCATTGGAGCACCTGACTCAACCACAAGGAGAAGAAA
CGATATTAACAAAGAGAGAAGAATGGCTTCTAAGGTCAAAGGCAAAAGAATCGTTGTTTG
AGATAATAATTATTATTCATCTCCAAATTTCAAAAGTTTCCTTTTTGATAGATTGAGAAA
AAAACAAAATTCCAAATTTTATCTTTTTGGTGGGTTATATGTTCTTTATTTGGTTGTTAT
GTGTATTGTACAAGAGAATTGACAAAACACAGAAATGAGAGGAATTAATTTACATTCTAT
GTAAATTATTTATTTGATCCTTATATTTATTGTTCTAGAAGCCTACGTCATTATTGTAAG
AGTTGACGTAATATAAATGTTTTTGAAATTGAAAATATGTATATTGGTATAGATGCCAAT
AAAAAT

Last Updated on 06.12.2018 16:11