Sequences from confirmation amplicon


Confirmation sequences for line 125G06 and hit At3g08850 are shown.
Line ID 125G06
Locus At3g08850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2691333
Primers used for PCR 14tv - 3144, show primer details
   
Sequence Name 27-K117118-0022-125-G06C-14tv
GenBank Accession LN498568 [GenBank]
Sequencing primer gene specific primer, 14tv: 5'-GTGTGGATATGGCTTAGGAACATT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K117118-0022-125-G06C-14tv
ATTTTGTTTGCATATTGCCATTTTAGCGTATTTATAGAATCTGGTTTGTTTCAGGTGTTA
CTTAGCCAGTGCCATCGGTTTCGTGCTCTTGTACTTCTTGGAAGATTTCTTGATATGGGT
TCATGGGCTGTGGATCTGGTATACTTCTTTAAATTTTAATTTCTCTCTCCCTTTGGCTTA
CACAGAGTTTCTCCTTTTTATCATGTATATGTCTTTACACGTGAAGTGAACATTCTTCTA
GGCCTTGTCTGTTGGAATATTCCCATATGTGCTGAAGCTTCTGCAAACAACAACGAATGA
GCTAAGACAGATCCTGGTTTTCATATGGACAAAAATTCTTGCACTTGACAAGGTAAATAT
CTGATGTTCCATGAGCTATTTTTTGGGAGGAAGATGGTGGTTTGCCATGCCTCTTTTTGT
TATTCTACAAATTGTAAATGGTAAAAAGAAAGAAAAAGAAAATGACCATCAAACACTGAT
AGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAAT
GAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCAT
CCCTTACGTCAGTGGAGATA
   
Sequence Name 05-K117117-0022-125-G06C-3144
GenBank Accession LN488523 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117117-0022-125-G06C-3144
CTTCGCAGACCCTTCCTCTATAAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCG
GGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAACTATCAGTGTTTGATGGTCATTTTCT
TTTTCTTTCTTTTTACCATTTACAATTTGTAGAATAACAAAAAGAGGCATGGCAAACCAC
CATCTTCCTCCCAAAAAATAGCTCATGGAACATCAGATATTTACCTTGTCAAGTGCAAGA
ATTTTTGTCCATATGAAAACCAGGATCTGTCTTAGCTCATTCGTTGTTGTTTGCAGAAGC
TTCAGCACATATGGGAATATTCCAACAGACAAGGCCTAGAAGAATGTTCACTTCACGTGT
AAAGACATATACATGATAAAAAGGAGAAACTCTGTGTAAGCCAAAGGGAGAGAGAAATTA
AAATTTAAAGAAGTATACCAGATCCACAGCCCATGAACCCATATCAAGAAATCTTCCAAG
AAGTACAAGAGCACGAAACCGATGGCACTGGCTAAGTAACACCTGAAACAAACCAGATTC
TATAAATACGCTAAAATGGCAATATGCAAACAAAATTATGATAGAACGTCCTGAAGGAAG
CCAAAGAATGTTCCTAAGCC

Last Updated on 06.12.2018 16:11