Sequences from confirmation amplicon


Confirmation sequences for line 125H07 and hit At4g33970 are shown.
Line ID 125H07
Locus At4g33970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16280987
Primers used for PCR 06dr - 8474, show primer details
   
Sequence Name 25-K110307-0022-125-H07C-06dr
GenBank Accession LN497491 [GenBank]
Sequencing primer gene specific primer, 06dr: 5'-ATTTTCAGACCCATTTAACACCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K110307-0022-125-H07C-06dr
GGCTACCCGGTGTGGTTTGTGCGCCAGCTCTTGATGATTCCGATGTCACGGTTGTAGCTG
GTGTTGACCTCAACGGTGCGGATATCGCAGGGCATTTGCCTGCTGAGCTTGGTTTGATGA
CAGATGTTGCAATGTTCCATTTGAATTCAAACCGGTTTTGTGGGATCATACCTAAAAGCT
TTGAGAAGCTAAAGCTAATGCACGAATTCGATGTTAGTAACAACCGTTTCGTTGGACCTT
TCCCTAACGTCGTTCTCTCTTGGCCTGATGTCAAATACTTCGACCTTAGGTTCAACGATT
TCGAAGGTCAAGTCCCTCCTGAGCTTTTCAAGAAAGAACTTGACGCCATTTTCTTGAACG
ACAATAGATTCACCTCGGTGATTCCTGAATCTCTAGGGGAATCTCCGGCCTCGGTTGTGA
CTTTTGCTAACAATAAATTCACCGGATGTATCCCTAAGAGTATCGGAAACATGAAGAATC
TCAACGAGATTGTCTTTATGGACAATGATTTAGGAGGTTGTTTCCCATCTGAAATCGGAA
AGTTATCGAATGTAACAATGATTATGATGGATCAATGAGTATCGGAAGTTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 09-K110306-0022-125-H07C-8409
GenBank Accession LN490229 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K110306-0022-125-H07C-8409
GCCATTTACAATTGAACTTCCGATACTCATTGATCCATCATAATCATTGTTACATTCGAT
AACTTTCCGATTTCAGATGGGAAACAACCTCCTAAATCATTGTCCATAAAGACAATCTCG
TTGAGATTCTTCATGTTTCCGATACTCTTAGGGATACATCCGGTGAATTTATTGTTAGCA
AAAGTCACAACCGAGGCCGGAGATTCCCCTAGAGATTCAGGAATCACCGAGGTGAATCTA
TTGTCGTTCAAGAAAATGGCGTCAAGTTCTTTCTTGAAAAGCTCAGGAGGGACTTGACCT
TCGAAATCGTTGAACCTAAGGTCGAAGTATTTGACATCAGGCCAAGAGAGAACGACGTTA
GGGAAAGGTCCAACGAAACGGTTGTTACTAACATCGAATTCGTGCATTAGCTTTAGCTTC
TCAAAGCTTTTAGGTATGATCCCACAAAACCGGTTTGAATTCAAATGGAACATTGCAACA
TCTGTCATCAAACCAAGCTCAGCAGGCAAATGCCCTGCGATATCCGCACCGTTGAGGTCA
ACACCAGCTACAACCGTGACATCGGAATCATCAAGAGCTGGCGCACAAACCACACCGGTG
TAGCCGCACACGTGTGGGCCATGCCAGTTCCCCGTGGTGTTAAATGGGTCTGAAAA

Last Updated on 06.12.2018 16:11